Publication detail

Effect of Acetaminophen on Intracellular Glutathione Level and Redox Status in Hepatocytes Isolated from Steatotic Rat Liver.

Year: 2011

Type of publication: ostatní - přednáška nebo poster

Name of source: Twenty years of in Vitro Toxicology: Achievements and Future Challenges

Publisher name: ISTISAN Congressi

Place: Rome

Page from-to: 93

Titles:

Language	Name	Abstract	Keywords
eng	Effect of Acetaminophen on Intracellular Glutathione Level and Redox Status in Hepatocytes Isolated from Steatotic Rat Liver.	Introduction: Acetaminophen (APAP) overdose is the most common cause of acute liver failure in humans. Non-alcoholic fatty liver disease (NAFLD) is the most frequent chronic liver disease in the world. The pathogenesis of NAFLD is not completely understood, but accumulating data suggest that oxidative stress and altered redox balance play a crucial role in its pathogenesis. Results: APAP causes more severe dose-dependent damage of steatotic hepatocytes (compared to non-steatotic cells) in primary culture as documented by increased release of lactate dehydrogenase (LDH) to culture medium and LDH leakage, decreased activity of cellular dehydrogenases (WST-1 test) and reduced albumin production (marker of hepatocytes function, ELISA). MDA production (TBARS) and ROS formation (DCFDA) were also significantly higher in steatotic hepatocytes treated with APAP. Conclusion: Our results indicate that steatotic hepatocytes exert higher sensitivity to the toxic action of APAP.

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