Publication detail

Assessment of glutathione reductase inhibition

Authors: Nýdlová Erika | Roušar Tomáš

Year: 2011

Type of publication: článek v odborném periodiku

Name of source: Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology

Publisher name: Univerzita Pardubice

Place: Pardubice

Page from-to: 77-83

Titles:

Language	Name	Abstract	Keywords
cze	Testování inhibice glutathionreduktasy	Glutathionreduktasa je kruciální enzym účastnící se celé řady nitrobuněčných pochodů. S ohledem na její roli v celé řadě procesů je kromě znalosti její aktivity nutné také charakterizovat její možné inhibitory.	Gutathion reduktáza;glutathion
eng	Assessment of glutathione reductase inhibition	Glutathione reductase is a crucial enzyme for maintaining of intracellular glutathione levels. This enzyme catalyzes the NADPH-dependent reduction of glutathione disulfide to the reduced form (GSH). The aim of our study was to estimate a possible inhibitory effect on glutathione reductase activity in the presence of two substances that cause glutathione depletion - ethacrynic acid and diethyl maleate. We also tested glutathione as a possible inhibitor. GR activity was determined using spectrophotometric method based on measurement of absorbance decline (lambda = 340 nm) due to oxidation of NADPH. We found dose dependent inhibitory effect through reduced glutathione – the presence of 10 mM GSH caused a decrease in enzyme activity by 60%. We conclude that our finding of inhibitory effect in presence of glutathione is of great importance since the GSH levels are very high in the cells.	Glutathione reductase;glutathione;spectrophotometry

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