Publication detail

Injectable hydrogel functionalized by nanofibres as a drug delivery system for skeletal tissues regeneration

Year: 2012

Type of publication: článek v odborném periodiku

Name of source: Journal of Tissue Engineering and Regenerative Medicine

Publisher name: Jonh Wiley & Sons, Inc.

Place: Hoboken

Page from-to: 35

Titles:

Language	Name	Abstract	Keywords
cze	Injektovatelné gely funkcionalizované s pomocí nanovláken určené k transportu léčiv napomáhajících regeneraci kostní tkáně	Hydrogely představují významnou skupinu materiálů používaných ve tkáňovém inženýrství, jejichž předností je schopnost efektivně vázat vodu. Mohou být aplikovány injekčně. V dané studii byly hydrogely na bázi fibrinu, kyseliny hyaluronové a kolagenu typu I použity jako skafoldní systémy pro růst mesenchymalních kmenových buněk.	polykaprolakton; nanokapsle; hydrogely; nosičové systémy
eng	Injectable hydrogel functionalized by nanofibres as a drug delivery system for skeletal tissues regeneration	Hydrogels are favorite materials in tissue engineering for its big potential to bond water. It enables good transport of nutrients and waste product and also homogenous distribution of cells. In addition, hydrogels can be injected directly to the defect and fill in defects of different shapes and sizes and subsequently can be polymerized chemically and physically. This material can also be enriched by bioactive molecules or particles and used as a drug delivery system. In our study, injectable hydrogels based on fibrin, hyaluronic acid (HA) and type I collagen were seeded with mesenchymal stem cells (MSCs) and tested. Moreover, the scaffolds were enriched with thrombocytes, as a source of native growth factors, and/or capsules from polycaprolactone (PCL) loaded with dexamethasone and ascorbic acid. The hydrogels were two-component and solidified after mixing in 37 °C. The first component contained fibrin and type I collagen, the second one comprised of thrombin, MSCs, HA and thrombocytes and/or PCL capsules. The cells were viable for the whole 14 days of experiment. It was shown, that cell proliferation and viability was improved by thrombocytes, but not by PCL capsules. Neither combination of both thrombocytes and capsules was better than the control. On the other hand, the presence of capsules prolonged the disintegration of samples. This way the biomechanical properties of hydrogels can be improved. Hydrogels based on fibrin, hyaluronic acid and type I collagen enriched by thrombocytes showed improved cell proliferation and viability in comparison to control. Presence of capsules in hydrogel enhanced biomechanical properties and prolonged scaffold degradation. Moreover, these particles can serve as a drug delivery system for stimulation of cell proliferation and differentiation. Further experiments are essential for adjustment of drug dose and release kinetics.	polycaprolactone; capsules; hydrogels; scaffold

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