Publication detail

Preparation of a Microcarrier Scaffold System for Cell Cultivation Using Cryogenic Grinding of Thin Layers of Polymer Fibers.

Year: 2012

Type of publication: článek ve sborníku

Name of source: Studentská konference Chemie je život 2012: sborník příspěvků

Publisher name: Vysoké učení technické v Brně

Place: Brno

Page from-to: 346-351

Titles:

Language	Name	Abstract	Keywords
cze	Příprava mikronosičového scafoldního systému pro kultivaci buněk s použitím kryogenního mletí těnkých vrstev polymerních vláken.	Příprava mikronosičového scafolfdního systému pro kultivaci buněk s použitím kryogenního mletí tenkých vrstev polymerních vláken. Prezentovaná práce se zaměřila na přípravu mikronosičového systému k pěstování buněk z elektrospinovaného poly-ε-caprolactone (PCL) kryogenním mletím.	mikronosičový; scafoldní systém; kultivace buněk; kryogenní mletí; polymerní vlákna
eng	Preparation of a Microcarrier Scaffold System for Cell Cultivation Using Cryogenic Grinding of Thin Layers of Polymer Fibers.	One of the major requirements in scaffold preparation is an interconnected porous structure that mimics the extracellular matrix. Electrospun layers of polymeric fibers provide the desired structural features. However, the sheet-like character requires an invasive administration. The presented work aimed to prepare an injectable microcarrier system for cell growth from an electrospun poly-ε-caprolactone (PCL) nano/micro-mesh using cryogenic grinding. The polymer mesh was submerged in a liquid medium and pulverized at the temperature of liquid nitrogen. The obtained particles were subsequently characterized by scanning electron microscopy and laser diffraction. The examination revealed the presence of six particle types differing in morphology and size (fibrous particles, fibrous fragments, aggregates with and without an internal fibrous structure, lamellae and nanoparticles). To maximize the yield of porous particles, the influence of the parameters of cryogenic grinding (type of the grinding solvent, sample storage conditions, weight of sample load and different types of grinding vials) on the produced suspension was investigated. The biocompatibility of the system was evaluated by a cell cultivation study with 3T3 fibroblasts. Cell adhesion, viability, and proliferation on the PCL microparticles were examined and the results demonstrated the applicability of these particles as a cell scaffolding material.	cryogenic grinding; poly-ε-caprolactone; nano/micro-mesh; microsystem; characterization

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