Skip to main content

Login for students

Login for employees

Publication detail

An assay of 4-hydroxy-trans-2-nonenal in human seminal plasma using a high performance liquid chromatography with fluorescence detection
Authors: Drábková Petra | Martínková Jiřina | Míková Veronika | Štramová Xenie | Kanďár Roman
Year: 2012
Type of publication: článek v odborném periodiku
Name of source: Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 17-28
Titles:
Language Name Abstract Keywords
cze Stanovení 4-hydroxy-trans-2-nonenalu v seminální plazmě vysoceúčinnou kapalinovou chromatografií s fluorescenční detekcí Byla optimalizována metoda pro stanovení 4-hydroxy-trans-2-nonenalu v seminální plazmě mužů s poruchami plodnosti. Po zkapalnění byly vzorky spermatu odstředěny a seminální plazma byla zmrazena při -80°C. 4-Hydroxy-trans-2-nonenal byl derivatizován s 1,3-cyklohexanedionem za vzniku fluoreskujícího alkylakridinového derivátu. Po derivatizaci byly vzorky precipitovány kyselinou chloristou a vzorek byl poté nadávkován na chromatografickou kolonu. Separace probíhala na obrácené fázi, efluent byl detekován fluorescenčním detektorem. Mobilní fáze byla směs ethanolu a deionizované vody. Variační koeficient byl pod 10 % a výtěžnost se pohybovala v rozmezí 62,0 % až 81,0 %. 4-hydroxy-trans-2-nonenal; seminální plazma; oxidační stres, lipoperoxidace
eng An assay of 4-hydroxy-trans-2-nonenal in human seminal plasma using a high performance liquid chromatography with fluorescence detection A method is described for the determination of 4-hydroxy-trans-2-nonenal in human seminal plasma. Semen samples were obtained from male partners of couples presenting for a fertility evaluation. After liquefaction, the samples were centrifuged and the seminal plasma was stored at –80 °C. 4-Hydroxy-trans-2-nonenal was derivatized with 1,3-cyclohexanedione to generate the fluorescent alkyl acridine derivative. After derivatization, seminal plasma proteins were precipitated with cold perchloric acid, and the supernatant was injected into the HPLC system. The separation was realized on an analytical reversed-phase column with fluorescence detection. The mixture of ethanol and deionized water was used as the mobile phase. The intra-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked seminal plasma were between 62.0 and 81.0 %. 4-hydroxy-trans-2-nonenal; human seminal plasma; oxidative stres, lipid peroxidation