Publication detail

Effect of natural substances on bacteria contaminating boar semen

Authors: Mazurová Jaroslava | Kukla Rudolf | Mosio Petra

Year: 2012

Type of publication: ostatní - článek ve sborníku

Name of source: IIIrd International Scientific Symposium for Ph.D. Students and Students of Agricultural Colleges

Publisher name: University of Technology and Life Sciences in Bydgoszcz

Place: Bydgoszcz

Page from-to: 194-195

Titles:

Language	Name	Abstract	Keywords
eng	Effect of natural substances on bacteria contaminating boar semen	INTRODUCTION The aim of our study was to determine minimal bactericidal concentration (MBC) of natural substances (Carvacrol, Eugenol, Thymol, Cnicin, Cymene, Gallic acid, Ethylgallate) and their mutual combination. MATERIAL AND METHODS Single substances or their combinations were dissolved in 24% ethanol solution in phosphate buffered saline. Bacterial strains including Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus hyicus, Proteus vulgaris and Escherichia coli were isolated from boar semen. RESULTS AND DISCUSSION The most effective natural extracts used as a pure component were Thymol (MBC 300 – 1200 µg/ml) and Cnicin (MBC 600 – 1200 µg/ml). Combinations Carvacrol + Cnicin (MBC 600 + 300 µg/ml, Thymol + Cnicin (MBC 600 + 300 µg/ml) and Thymol + Gallic acid (MBC 300 + 600 µg/ml) were the most effective combinations used. CONCLUSSIONS The increasing number of resistant microorganisms is consequence of frequent antibiotic use not merely for therapeutic purposes. One of possible approaches to solution of this important problem is utilization of natural extracts as a source of antimicrobial compounds.	Effect; natural; substances; bacteria; contaminating; boar; semen

Search