Publication detail

Biofunctionalization of nanofibers and particles.

Authors: Slováková Marcela

Year: 2013

Type of publication: článek ve sborníku

Name of source: Modern diagnostic methods for human diseases

Publisher name: Transilvania University Press

Place: Brasov

Page from-to: 119-126

Titles:

Language	Name	Abstract	Keywords
cze	Biofunkcionalizace nanovláken a částic	V příspěvku jsou popsány strategie pro kovalentní imobilizaci proteinů na nosiče jako nanovlákna a sférické částice. Vyvinuté bioreaktory byly využity k detekci, izolaci, ke specifickému štěpení vybranými proteázami a fosforylaci kinázami molekul a analytů, jako např. protilátek, markerů chorob, peptidů.	Biofunkcionalizace; nanovlákna; částice
eng	Biofunctionalization of nanofibers and particles.	In the paper the immobilization strategy, carriers as nanofibers and spheric particles are described. Developed reactors were applied for specific detection or isolation of different targets, e.g. antibodies, disease markers, peptides; specific digestion by selected proteases, e.g. trypsin, proteinase K, papain, Arg-C (Clostripain), Lys-C; and phosphorylation by kinases. Methods of protein grafting are preferably covalent, due to advantageous attributes. In some cases a special covalent methods were used, so called oriented immobilizations, for ensuring the accessible orientation of a biomolecule active site. Development and applications of these reactors were intended for diagnostic, biotechnology and medicinal research.	Biofunctionalization; nanofibers; particles.

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