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Combined Effects of Mobile Phase Composition and Temperature on the Retention of Phenolic Antioxidants on an Octylsilica Polydentate Column
Authors: Jandera Pavel | Vyňuchalová Kateřina | Nečilová Kateřina
Year: 2013
Type of publication: článek v odborném periodiku
Name of source: Journal of Chromatography A
Publisher name: Elsevier Science BV
Place: Amsterdam
Page from-to: 49-58
Titles:
Language Name Abstract Keywords
cze Kombinované účinky složení mobilní fáze a teploty na retenci fenolických antioxidantů na octylsilica polydentátátové koloně. Kombinované účinky teploty a složení mobilní fáze na retenci a selektivitu separace fenolických kyselin a flavonoidů. Sloučeniny byly studovány v kapalinové chromatografie na polydentátní Blaze C - 8 křemičité koloně . Teplotní vliv na retencií může být popsán van't Hoff rovnicí . Dobrá linearita Ink proti 1 / T znamená, že zadržování je řízeno jediným mechanismem. Entalpické a entropické příspěvky k zadržování byly vypočteny z regresních přímek. Semi - empirické modely popisují současné účinky teploty a objemového zlomku organického rozpouštědla v mobilní fázi . Polydentátová kolona, fenolické kyseliny, gradientová eluce, vlivy teploty
eng Combined Effects of Mobile Phase Composition and Temperature on the Retention of Phenolic Antioxidants on an Octylsilica Polydentate Column Combined effects of temperature and mobile-phase composition on retention and separation selectivity of phenolic acids and flavonoid compounds were studied in liquid chromatography on a polydentate Blaze C-8 silica based column. The temperature effects on the retention can be described by van't Hoff equation. Good linearity of Ink versus 1/T graphs indicates that the retention is controlled by a single mechanism in the mobile phase and temperature range studied. Enthalpic and entropic contributions to the retention were calculated from the regression lines. Generally, enthalpic contributions control the retention at lower temperatures and in mobile phases with lower concentrations of methanol in water. Semi-empirical retention models describe the simultaneous effects of temperature and the volume fraction of the organic solvent in the mobile phase. Using the linear free energy-retention model, selective dipolarity/polarizability, hydrogen-bond donor, hydrogen-bond acceptor and molecular size contributions to retention were estimated at various mobile phase compositions and temperatures. In addition to mobile phase gradients, temperature programming can be used to reduce separation times. polydentate column; phenolic compounds; gradient elution; temperature effects

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