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Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns
Authors: Vyňuchalová Kateřina | Jandera Pavel
Year: 2013
Type of publication: článek v odborném periodiku
Name of source: Journal of Chromatography A
Publisher name: Elsevier Science BV
Place: Amsterdam
Page from-to: 37-45
Titles:
Language Name Abstract Keywords
cze Možnosti predikce retence v rychlém gradientu kapalinové chromatografie. Část 1: Srovnání separace na plně porézních, neporézních a monolitických kolonách Vzhledem ke stále rostoucímu významu krátké doby analýzy v moderní HPLC praxi se rychlé gradienty stávají široce použitelné, jak v jednorozměrných a dvourozměrných separací, a to zejména ve druhé dimenzi, kde má rychlost separace prvořadý význam. Reálnost těchto přístupů byla prokázána v konvenční kapalinové chromatografií a komerční software je pro tento účel k dispozici. V této práci byla studována gradientních predikce z isokratických naměřených dat pouze na dvou koncentracích mobilní fáze pro alkylbenzenů , fenylmočoviny a triazinových pesticidů na pěti kolonách různých délek: dvě z nich monolitické a tři s částicemi o různé velikosti . Rychlé gradienty; predikce; píková kapacita, pesticidy
eng Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns Due to ever increasing importance of short analysis times in modern HPLC practice, fast gradient elution is becoming widely used both in one-dimensional and two-dimensional separations, especially in the second dimension where the speed of separation is of primary importance. For method development and optimization, prediction of retention in gradient elution from the isocratic data is very important. This is principally possible using the well-established theory of gradient elution, especially for reversed-phase separations. Feasibility of these approaches has been proven in conventional liquid chromatography and commercial prediction software is available for this purpose. However, fast gradient chromatography employing short columns and gradient times in between 1 and 2 min or even less, impose additional stringent demands on the accuracy of prediction, due to rapid changes in mobile phase composition and increased role of extra-column contributions and instrumental dwell volume on the retention. In the present work, possibilities of prediction of the gradient elution times from the isocratic data measured only at two mobile phase concentrations were investigated in fast chromatography of alkylbenzenes, phenylurea and triazine pesticides on five columns differing in lengths: two monolithic ones and three packed with particles of different size. In the second dimension, gradient times are strongly restricted by the short cycle frequency. The effects of the flow-rate on the elution times, on the accuracy of their prediction and on peak capacity were studied under time constraint conditions. Gradient theory can be useful for the optimization of fast gradients. fast gradient elution; prediction of retention; peak capacity; pesticides

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