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Retention behavior of isomeric Triacylglycerols in Silver-ion HPLC: Effects of Mobile Phase Composition and Temperature
Authors: Lísa Miroslav | Denev Rumen | Holčapek Michal
Year: 2013
Type of publication: článek v odborném periodiku
Name of source: Journal of Separation Science
Publisher name: Wiley-VCH
Place: Weinheim
Page from-to: 2888-2900
Titles:
Language Name Abstract Keywords
cze Retenční chování izomerů triacylglycerolů v argentační HPLC: efekt složení mobilní fáze a teploty V této práci je porovnáváno retenční chování všech známých izomerů triacylglycerolů pomocí argentační HPLC v mobilních fázích založených na hexanu a dichlormethanu. argentační HPLC; polohové izomery dvojných vazeb; regioizomery; triacylglycerol; lipidy; hmotnostní spektrometrie
eng Retention behavior of isomeric Triacylglycerols in Silver-ion HPLC: Effects of Mobile Phase Composition and Temperature A systematic study of the retention behavior of isomeric triacylglycerols (TGs) in silver-ion HPLC on a ChromSpher Lipids column has been performed between 10 to 40 degrees C using the most widespread hexane- and dichloromethane-based mobile phases. The randomization of mono-acyl TG standards and the random esterification of glycerol with fatty acids are employed to produce mixtures of TG isomers. The mobile phase composition has no influence on the general retention pattern, but significant differences in the retention order of double bond (DB) positional isomers in hexane and dichloromethane mobile phases are described and compared with the previous literature data. Saturated TGs with fatty acyl chain length from C7:0 to C22:0 are partially separated using the hexane mobile phase but not at all with the dichloromethane mobile phase. The hexane mobile phase enables at least partial resolution of TG regioisomers with up to seven DBs, while the resolution of only ALA/AAL and ALnA/AALn isomers is achieved with the dichloromethane mobile phase. The effect of temperature differs significantly depending on the mobile phase composition. Retention times of TGs increase with increasing temperature in the hexane mobile phase, while an opposite effect is observed for the dichloromethane mobile phase. Double bond positional isomers; Regioisomers; Silver-ion HPLC; Trans isomers; Triacylglycerol; mass spectrometry

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