Publication detail

New Method for the Determination of the Half Inhibition Concentration (IC50) of Cholinesterase Inhibitors

Authors: Kovářová Markéta | Komers Karel | Štěpánková Šárka | Pařík Patrik | Čegan Alexander

Year: 2013

Type of publication: článek v odborném periodiku

Name of source: Zeitschrift fuer Naturforschung C-A Journal of Biosciences

Publisher name: Verlag der Zeitschrift für Naturforschung

Place: Tübingen

Page from-to: 133-138

Titles:

Language	Name	Abstract	Keywords
cze	Nová metoda stanovení poloviční inhibiční koncentrace (IC50) cholinesterázových inhibitorů	Byla popsána nová a jednoduchá analytická metoda pro stanovení IC50 hodnot inhibitorů hydrolýzy acetylcholinu a acetylthiocholinu pomocí choliesteráz. Metoda je založena na monitorování časové závislosti pH během neinhibované a inhibované reakce. Potřebný je pouze pH-metr s vhodnou měřicí celou a malý termostatovaný míchaný vsádkový reaktor.	cholinesteráza; inhibice; IC50; stanovení
eng	New Method for the Determination of the Half Inhibition Concentration (IC50) of Cholinesterase Inhibitors	A new and simple analytical method is described for the determination of the IC50 values of the inhibitors of the hydrolysis of acetylcholine (ACh) or acetylthiocholine (ATCh) by cholinesterases. The method is based on monitoring the time course of the pH value during the uninhibited and inhibited reaction. It requires only a pH meter with a suitable pH measuring cell and a small thermostated stirred batch reactor. The method has been validated for twelve different types of cholinesterase inhibitors. The determined IC50 values are comparable to those obtained by independent, more complicated, and expensive methods (Ellman's and pH-stat).	cholinesterase Inhibition; IC50; determination

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