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Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection
Authors: Drábková Petra | Bencová Soňa | Štramová Xenie | Žáková Pavla | Kanďár Roman
Year: 2013
Type of publication: článek v odborném periodiku
Name of source: Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 25-38
Titles:
Language Name Abstract Keywords
cze Stanovení adrenalinu, noradrenalinu a dopaminu v lidské moči metodou vysokoúčinné kapalinové chromatografie s coulometrickou detekcí Byla vyvinuta relativně jednoduchá metoda pro simultánní stanovení adrenalinu, noradrenalinu a dopaminu v moči. Použili jsme vysokoúčinnou kapalinovou chromatografii (HPLC) s coulometrickou elektrochemickou detekcí. Vzorky moči byly ošetřeny extrakcí na tuhé fázi a poté přímo dávkovány do HPLC systému. Katecholaminy byly separovány na koloně Discovery HS C18, 250 x 4 mm, 5 um. Analytické parametry metody byly vyhovující: přesnost v sérii a mezi sériemi byla pod 10 %. Výtěžnost metody byla 78,2-88,0% pro adrenalin, 80,0-93,0% pro noradrenalin a 96,4-107,2% pro dopamin. Linearita byla dosažena v rozmezí 2-200 ug l-1, s detekčním limitem 0,6 ug l-1 pro adrenalin, 5-500 ug l-1, s detekčním limitem 1,5 ug l-1 pro noradrenalin a 20-2000 ug l-1, s detekčním limitem 6 ug l-1 pro dopamin. adrenalin; noradrenalin; dopamin; kapalinová chromatografie; coulometrická elektrochemická detekce; extrakce na tuhé fázi
eng Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection A relative simple method for the simultaneous determination of epinephrine, norepinephrine and dopamine in urine is presented. We have used a high-performance liquid chromatography (HPLC) with coulometric electrochemical detection. Urine samples pretreatment by a solid-phase extraction were injected directly into the HPLC system and were eluted isocratically on a Discovery HS C18, 250 x 4 mm i.d., 5 um, analytical column. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked urine samples were at intervals 78.2-88.0% for epinephrine, 80.0-93.0% for norepinephrine, and 96.4-107.2% for dopamine. The linear range is 2-200 ug l-1, with a detection limit of 0.6 ug l-1 (signal-to-noise ratio = 3) for epinephrine, 5-500 ug l-1, with a detection limit of 1.5 ug l-1 for norepinephrine, and 20-2000 ug l-1, with a detection limit of 6 ug l-1 for dopamine. epinephrine; norepinephrine; dopamine; liquid chromatography; coulometric electrochemical detection; solid-phase extraction

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