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Electrocatalytic Oxidation of NADH by Poly Toluidine Blue Multi Walled Carbon Nanotubes Modified Glassy Carbon Electrode
Authors: Vu Dai Long | Červenka Libor
Year: 2013
Type of publication: článek v odborném periodiku
Name of source: Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 15-24
Titles:
Language Name Abstract Keywords
cze Elektrokatalytická oxidace NADH uhlíkovou skelnou elektrodou modifikovanou vícestěnnými uhlíkovými nanovlákny s elektroaktivním filmem toluidinové modři V této práci byla vytvořena uhlíková skelná elektroda se stabilním elektroaktivním filmem poly-toluidinové modři na povrchu vícestěnných uhlíkových vláken s využitím cyklické voltametrie v B-P pufru (pH 9,0) obsahující 0,1 M NaNO3 v potenciálovém rozsahu -0,5 až +1,0 V vs. Ag/AgCl. Takto připravená elektroda vykazovala dobrý elektrokatalytický efekt pro oxidaci ß-Nicotinamide Adenine Dinucleotidu (NADH) ve fosfátovém pufru (pH 7,0) s obsahem 0,1 M KCl. Výsledný proud oxidace byl za optimálních podmínek lineárně závislý na koncentraci NADH. Kalibrační křivka byla získána v rozsahu koncentrací 0,1-100 mikroM s detekčním limitem 0,04 mikroM.
eng Electrocatalytic Oxidation of NADH by Poly Toluidine Blue Multi Walled Carbon Nanotubes Modified Glassy Carbon Electrode In this study, a stable electroactive thin film of poly Toluidine blue (PTB) was fabricated at the surface of multi-walled carbon nanotube (MWCNT) modified glassy carbon electrode (GCE) by recording successive cyclic voltammograms of 0.25 mM Toluidine blue (TB) in a Britton Robinson bufer solution (pH 9.0) containing 0.1 M NaNO3 at the potential range from -0.5 to +1.0 V vs. Ag/AgCl. This PTB/MWCNT/GCE exhibited a good electrocatalytic effect towards the oxidation of reduced ß-Nicotinamide Adenine Dinucleotide (NADH) in phosphate buffer solution (PBS) pH 7.0 containing 0.1 M KCl at a reduced overpotential as well as an increased peak current compared with those at a bare electrode. The currents obtained from the amperometric measurements at optimum conditions (supporting electrolyte: pH 7.0 PBS containing 0.1 M KCl and constant applied potential: +150 mV vs. Ag/AgCl) were linearly correlated with the NADH concentration. The calibration curve was obtained for NADH concentrations in a range of 0.1-100 microM. The detection limit was found to be 0.04 micoM for amperometric method. electrocatalytic oxidation; modified electrode; NADH; amperometric detection; Toluidine blue; voltammetry

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