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Electrochemical Behavior of Mixture of Phosphatidylcholine and Cholesterol in Model Phospholipid Membranes Using Impedance Spectroskopy
Authors: Nováková Kateřina | Navrátil Tomáš | Šestáková Ivana | Mareček Vladimír | Chýlková Jaromíra
Year: 2013
Type of publication: článek ve sborníku
Name of source: XIII. Pracovní setkání fyzikálních chemiků a elektrochemiků (13th workshop of Physical Chemists and Electrochemists)
Publisher name: Mendelova univerzita v Brně
Place: Brno
Page from-to: 128-129
Titles:
Language Name Abstract Keywords
cze Elektrochemické chování směsi fosfatidylcholinu a cholesterolu v modelové fosfolipidické membráně s využitím spektroskopie V této práci bylo zkoumáno elektrochemické chování směsi fosfatidylcholinu a cholesterolu v modelové fosfolipidické membráně s využitím spektroskopie (electrochemical impedance spectroscopy - EIS). voltametrie; modelová fosfolipidická membrána
eng Electrochemical Behavior of Mixture of Phosphatidylcholine and Cholesterol in Model Phospholipid Membranes Using Impedance Spectroskopy Biological membrane plays one of the most important roles in living cells. The different model of phospholipid membranes (PLMs) are used for better understanding of properties and functionality of real membranes. The mechanism of transporting processes and the properties of membranes can be studied on planar phospholipid bilayers [1,2]. This type of model membrane was utilized in research realized by our group [3]. Electrochemical behavior of phosphatidylcholine-cholesterol mixture in the form of PLMs was investigated by electrochemical impedance spectroscopy (EIS). For research of planar model biomembranes and for characterization of the electrochemical properties, EIS seems to be very suitable method. Phosphatidylcholine (lecithin) and cholesterol are essential for living cells and, therefore, they were chosen for presented experiments. Different ratios between lecithin and cholesterol were tested on different types of polycarbonate substrates. The influence of time and of temperature on creation of PLMs in presence of cholesterol was studied. voltammetry; model phospholipid membrane

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