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Chemical Composition of Bioactive Alkaloid Extracts from Some Narcissus Species and Varieties and their Biological Activity
Authors: Havlasová Jana | Safratova Marcela | Siatka Tomas | Štěpánková Šárka | Novak Zdenek | Locarek Miroslav | Opletal Lubomir | Hrabinova Martina | Jun Daniel | Benesova Nina | Kunes Jiri | Cahlikova Lucie
Year: 2014
Type of publication: článek v odborném periodiku
Name of source: Natural Product Communications
Publisher name: Natural Products Inc.
Place: Westerville
Page from-to: 1151-1155
Titles:
Language Name Abstract Keywords
cze Chemické složení bioaktivních alkaloidních extraktů z vybraných rostlin druhu Narcissus a jejich biologická aktivita Alkaloidní extrakty osmi rostlin rodu Narcissus (Amaryllidaceae) byly studovány s ohledem na jejich inhibiční aktivitu vůči acetylcholinesterase (HuAChE) s butyrylcholinesterase (HuBChE). Pomocí GC/MS bylo určeno 30 alkaloidů, a 25 z nich bylo identifikováno pomocí hmotnostních spekter, retenčních časů a retenčních indexů. Slibná inhibiční aktivita vůči HuAChE byla prokázána u šesti druhů narcisů. Inhibiční účinnost alkaloidů z N. jonquila cv. Double Campernelle a N. nanus cv. Elka vůči HuBChE byla 24.1 +/- 1.9 mu g/mL and 25.1 +/- 1.8 mu g/mL. Dva alkaloidy byly izolovány v čisté formě preparativní TLC a identifikovány jako alkaloid galanthaminovéo typu narwedin a alkaloid lykorinového typu incartin. Obě sloučeniny byly testovány na jejich biologickou aktivitu. Ukázaly se jako neaktivní při inhibici HuAChE/HuBChE, ale vykázaly slibnou prolyl oligopeptidasovou inhibiční aktivitu s hodnotami IC50 0.95 +/- 0.12 mM a 0.91 +/- 0.09 mM.
eng Chemical Composition of Bioactive Alkaloid Extracts from Some Narcissus Species and Varieties and their Biological Activity Alkaloid extracts of eight Narcissus (Amaryllidaceae) species and varieties were studied with respect to their acetylcholinesterase (HuAChE) and butyrylcholinesterase (HuBuChE) inhibitory activity and alkaloid patterns. Thirty alkaloids were determined by GC/MS, and twenty-five of them identified from their mass spectra, retention times and retention indexes. Promising HuAChE inhibition activity was demonstrated by six Narcissus taxa and HuBuChE inhibition by N. jonquila cv. Double Campernelle and N. nanus cv. Elka with IC50 values of 24.1 +/- 1.9 mu g/mL and 25.1 +/- 1.8 mu g/mL, respectively. Two alkaloids were isolated in pure form using preparative TLC and identified as the galanthamine type alkaloid narwedine and the lycorine type alkaloid incartine. Both compounds were tested for their biological activity. They were considered inactive in HuAChE/HuBuChE assays, but showed promising prolyl oligopeptidase inhibition activities with IC50 values of 0.95 +/- 0.12 mM and 0.91 +/- 0.09 mM, respectively. Narcissus; GC/MS; Alkaloids; Acetylcholinesterase; Butyrylcholinesterase; Prolyl oligopeptidase

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