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Determination of the polyphenolic content of a Capsicum annuum L. extract by liquid chromatography coupled to photodiode array and mass spectrometry detection and evaluation of its biological activity
Authors: Mokhtar Meriem | Soukup Jan | Donato Paola | Cacciola Francesco | Riazi Ali | Jandera Pavel | Dugo Paola | Mondello Luigi
Year: 2015
Type of publication: článek v odborném periodiku
Name of source: Journal of Separation Science
Publisher name: Wiley-VCH
Place: Weinheim
Page from-to: 171-178
Titles:
Language Name Abstract Keywords
cze Stanovení polyfenolických látek a biologické aktivity v Capsicum annuum L. pomocí kapalinové chromatografie spojené s fotodiodovým polem a hmotnostní spektrometrií Uvedená studie pojednává o stanovení polyfenolů v zelených paprikách pocházejících z Alžírska. Celkový obsah polyfenolů byl 1,373 mg ekvivalentu ke kyselině gallové a 0,098 mg quercetinu. Stanovení úplného polyfenolického profilu v extraktu paprik bylo provedeno pomocí kapalinové chromatografies RP-amidovou kolonou s UV a hmotnostní detekcí s ionizací elektrosprejem. V paprikách jsme identifikovali celkem 18 látek, z nichž pět bylo objeveno poprvé. antimikrobiální aktivita;antioxidační aktivita;Capsicum annum;kapalinová chromatografie;polyfenoly
eng Determination of the polyphenolic content of a Capsicum annuum L. extract by liquid chromatography coupled to photodiode array and mass spectrometry detection and evaluation of its biological activity The present study was aimed to investigate the polyphenolic profile of a pepper (Capsicum annuum L.) extract from Algeria and evaluate its biological activity. The total polyphenol content of the extract was determined as 1.373 mg of gallic acid equivalents (±0.0046), whereas the flavonoids were determined as 0.098 mg of quercetin (±0.0015). The determination of the complete polyphenolic profile of the extract was achieved by liquid chromatography with an RP-amide column in combination with photodiode array and mass spectrometry detection through an electrospray ionization interface. A total of 18 compounds were identified, of which five were reported for the first time in the sample tested. Quercetin rhamnoside was the most abundant compound (82.6 g/g of fresh pepper) followed by quercetin glucoside (19.86 g/g). The antioxidant activity and antimicrobial effects were also determined. For the antimicrobial tests assessed against Gram-positive and Gram-negative bacteria, kaempferol showed the strongest inhibitory effect followed by quercetin and caffeic acids. In the study of the cytotoxicity of the extract, the cancer cells (U937) were more affected than the normal cells (peripheral blood mononucleated cells), with more than 62% inhibition at the highest concentration. Antimicrobial activity;Antioxidant activity;Capsicum annuum L;Liquid chromatography;Polyphenols

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