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Surface Modification of Hypercrosslinked Monolithic Stationary Phases
Authors: Škeříková Veronika | Janků Simona | Urban Jiří
Year: 2014
Type of publication: ostatní - článek ve sborníku
Name of source: 20th International Symposium on Separation Sciences (ISSS 2014) : book of proceedings
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 71-71
Titles:
Language Name Abstract Keywords
cze Modifikace povrchu hypercroslinkovaných monolitických stacionárních fází Hypercroslinkování je pospolymerizační modifikace pocrchu která umožňuje vzniknout malým pórům a zepšuje separaci malých molekul. V naší práci byl povrch zesítěn diaminooktanem pomocí nukleofilní substituce. Nezreagované chlormetylové skupiny pak byly modifikovány Taurinem. Tak vznikla stacionární fáze vhodná pro separace chromatogrofií hydrofilních interakcí i chromatografií v systému s obrácenými fázemi. Hypercrosslinkování; Modifikace povrchu; Taurin; HILIC
eng Surface Modification of Hypercrosslinked Monolithic Stationary Phases Hypercrosslinking is a post-polymerization modification that forms a thin layer of small pores at the monolithic surface and allows fast and efficient isocratic separations of small molecules. Generally, Friedel-Crafts alkylation of reactive chloromethyl groups is used to modify surface of the monolithic phase. In this work we have used a different approach to prepare hypercrosslinked monolithic stationary phases. The swollen poly(styrene-co-chloromethyl styrene-co-divinylbenzene) monoliths have been hypercrosslinked by nucleophilic substitution of chloromethyl groups with diaminooctane. Then, residual chloromethyl groups have been modified by 2-aminoethanesulfonic acid (taurine) to prepare monolithic stationary phases applicable in hydrophilic interaction liquid chromatography. This protocol allows to prepare a stationary phase that combines non-polar monolithic skeleton with polar functional groups on the surface and provides dual retention mechanism tunable by the composition of the mobile phase. We have applied the prepared monolithic capillary columns in the separation of phenolic compounds and flavonoids in wine, beer and other beverages. Hypercrosslinking; Surface modification; Taurin; HILIC

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