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Identification of Combined Conjugation of Nabumetone Phase I Metabolites with Glucuronic Acid nad Glycine in Minipig Biotransformation Using Coupling High-performance Liquid Chromatography with Electrospray Ionization Mass Spectrometry
Authors: Česlová Lenka | Holčapek Michal | Nobilis Milan
Year: 2014
Type of publication: článek v odborném periodiku
Name of source: Journal of Pharmaceutical and Biomedical Analysis
Publisher name: Pergamon-Elsevier Science Ltd.
Place: Oxford
Page from-to: 221-224
Titles:
Language Name Abstract Keywords
cze Identifikace kombinovaných konjugátů metabolitů I. fáze s glukuronovou kyselinou a glycinem pomocí HPLC/ESI-MS u biotransformace miniprasete HPLC spojená s ESI-MS byla použita pro analýzu metabolitů nabumetonu během biotransformace u miniprasat. Identifikace byla provedena na základě interpretace MS a MS/MS spekter při snímání kladných a záporných iontů. Některé metabolity I. fáze byly konjugovány jak s glukuronovou kyselinou tak s glycinem, což je nezvyklý typ metabolitů II. fáze, který dosud nebyl publikován. Tento typ metabolitů byl nalezen v tenkém střevě miniprasat. metabolismus léčiv; konfugace s glukuronovou kyselinou; konfugace s glycinem; HPLC/MS; tandemová hmotnostní spektrometrie
eng Identification of Combined Conjugation of Nabumetone Phase I Metabolites with Glucuronic Acid nad Glycine in Minipig Biotransformation Using Coupling High-performance Liquid Chromatography with Electrospray Ionization Mass Spectrometry High-performance liquid chromatography (HPLC) coupled with electrospray ionization mass spectrometry (ESI-MS) was applied for the analysis of nabumetone metabolites during the biotransformation in minipigs. In addition to known phase I metabolites, the identification of phase II metabolites was achieved on the basis of their full-scan mass spectra and subsequent MSn analysis using both positive-ion and negative-ion ESI mode. Some phase I metabolites are conjugated with both glucuronide acid and glycine, which is quite unusual type of phase II metabolite not presented so far for nabumetone. These metabolites were found in small intestine content, but they were absent in minipigs urine. drug metabolites; conjugation with glucuronide acid; conjugation with glycine; HPLC/MS; tandem mass spectrometry

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