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Determination of Insulin Using a Pretreated Pencil Graphite Electrode
Authors: Vu Dai Long | Ertek Bensu | Červenka Libor | Dilgin Yusuf
Year: 2014
Type of publication: článek ve sborníku
Name of source: Sensing in Electroanalysis Vol. 8
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 305-313
Titles:
Language Name Abstract Keywords
cze Stanovení inzulínu s využitím modifikované mikrotužkové elektrody Mikrotužková elektroda modifikována chronoamperometricky ve fosfátovém pufru (pH 7,0) byla použita pro detekci inzulínu metodou diferenční pulsní voltametrie s anodickou prekoncentací analytu. V rámci párce byly testovány podmínky analýzy (pH pracovního pufru, potenciál akumulace a čas akumulace) pro zlepšení signálu oxidace inzulínu. Při akumulačním poteniálu 400 mV (vs. Ag/AgCl/3,0M KCl), 60 s akumulaci při pH 7,0 byla získaná lineární odezva oxidačního proudu na koncentraci inzulínu v rozsahu 5,0-200 nM s limitem detekce 2,0 nM.
eng Determination of Insulin Using a Pretreated Pencil Graphite Electrode In this paper, pretreated pencil graphite electrode (Pre-PGE) was used for the determination of insulin by anodic stripping differential pulse voltammetry (ASDPV). The influences of the pretreatment procedures, pH values, accumulation potential and time on the signal enhancement of insulin were optimized, and a novel electrochemical method for the determination of insulin was described. The currents obtained from ASDPV measurements at optimum conditions (phosphate buffer solution pH 7.0), accumulation time (60 seconds), applied potential 400 mV vs. Ag/AgCl/3.0 mol L-1 KCl) were linearly correlated with the concentration of insulin. Calibration curve was obtained for insulin concentrations in the range of 5.0-200.0×10-9 mol L-1 .The limit of detection was found to be 2.0×10-9 mol L-1. Pencil graphite electrode; Insulin; Anodic stripping pulse voltammetry

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