Publication detail

Development of Micellar Electrokinetic Chromatographic Method for Determination of Polyphenols in Elder Infusions

Authors: Váňová Jana | Česla Petr | Česlová Lenka | Fischer Jan

Year: 2014

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Development of Micellar Electrokinetic Chromatographic Method for Determination of Polyphenols in Elder Infusions	A method for determination of 18 flavonoid aglycones, glycosides and phenolic acids using micellar electrokinetic chromatography has been developed. Sodium decyl sulphate was used as pseudostationary phase at the concentration of 36 mmol L–1 and addition of cyclodextrins up to the 5 mmol L–1 was tested for improvement of the separation of isomeric compounds. In the electrolyte containing heptakis(2,6-di-O-methyl)-beta-cyclodextrin, three isomers of chlorogenic acid (chlorogenic, neo- and cryptochlorogenic acid) were successfully baseline separated. The quantitative parameters of the method were determined including limits of quantification, which were in the range of 0.15–1.4 ug mL–1, together with the linearity of calibration curves. The method was successfully applied for the analyses of water infusions of elderflower samples and the statistically significant differences were found between the concentrations of polyphenols depending on the sample harvest location.

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