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Electrochemical mechanism and sensitive assay of antiretroviral drug Abacavir in biological sample using multiwalled carbon nanotube modified pyrolytic graphite electrode
Authors: Ashrafi Amirmansoor | Kurbanoglu Sevinc | Vytřas Karel | Uslu Bengi | Ozkan Sibel A.
Year: 2014
Type of publication: článek v odborném periodiku
Name of source: Journal of Electroanalytical Chemistry
Publisher name: Elsevier Science SA
Place: Lausanne
Page from-to: 178-184
Titles:
Language Name Abstract Keywords
cze Elektrochemický mechanismus a test citlivosti antiretrovirálního léčiva Abacaviru v biologickém vzorku pomocí pyrolytické grafitové elektrody modifikované uhlíkovými nanotrubičkami Byla vyvinuta elektroda z pyrolytického grafitu s uhlíkovými nanotrubičkami (MWCNT / EPPGE), která byla využita pro stanovení antiretrovirového léčiva Abacaviru pomocí diferenciální pulsní adsorptivní stripping voltametrie (DPAdSV). Na cyklickém voltamogramu bylo možno pozorovat dva oxidační píky umístěné při potenciálech 0,91 a 1,10 V. Oxidační potenciály byly ovlivněn hodnotou pH, neboť elektrochemické reakce se účastní vodíkové ionty. Ukázalo se, že akumulace na povrchu elektrody je způsobena kombinací difúzních a adsorpčních procesů. Na základě získaných dat byl navržen oxidační mechanismus. Závislost proudové odezvy na koncentraci Abacaviru byla lineární v rozmezí 1 × 10-7 – 2 × 10-5 M. Metoda byla plně validována s ohledem na selektivitu, citlivost, přesnost a správnost. Abacavir byl navrženým způsobem stanoven také ve farmaceutických lékových formách a vzorcích lidského séra.
eng Electrochemical mechanism and sensitive assay of antiretroviral drug Abacavir in biological sample using multiwalled carbon nanotube modified pyrolytic graphite electrode Multiwalled carbon nanotube modified edge plane pyrolytic graphite electrode (MWCNT/EPPGE) was developed and implemented for the determination of antiretroviral drug Abacavir using differential pulse adsorptive stripping voltammetry (DP AdSV). Cyclic voltammogram showed two oxidation peaks located at +0.91 V and +1.10 V on (MWCNT/EPPGE). Dependency of peak potential and peak current on pH was investigated as details. The oxidation potentials affected by the pH indicating protons are involved in the electrochemical oxidation of Abacavir. Mass transport to the electrode surface was found as a combination of diffusion and adsorption which was concluded by studying the potential scan rate dependency of both peak currents. The oxidation mechanism was proposed using the obtained data and discussed. A large enhancement in both of the peak currents was observed with applying an accumulation step presenting the effect of adsorption. Peak currents showed a linear relation upon the Abacavir concentration within a range of 1 x 10(-7)-2 x 10(-5) M with (r = 0.999) for both peak responses. The method was fully validated related with selectivity, sensitivity, precision and accuracy studies. Abacavir was also determined with the proposed method in its pharmaceutical dosage forms and human serum samples. No interferences from the excipients of the dosage form or endogenous substances from biological material were found. Abacavir; Oxidation mechanism; Multiwalled carbon nanotube; Edge plane pyrolytic graphite electrode; Determination; Adsorptive stripping voltammetry

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