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DETERMINATION OF THE CONTENT OF ESSENTIAL FATTY ACIDS IN EDTA PLASMA TYPE 2 DIABETICS
Authors: Líbalová Martina | Laštovička Petr | Čermák Tomáš | Čegan Alexander
Year: 2014
Type of publication: ostatní - článek ve sborníku
Name of source: YISAC 2014 Book of Abstracts
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 42-42
Titles:
Language Name Abstract Keywords
eng DETERMINATION OF THE CONTENT OF ESSENTIAL FATTY ACIDS IN EDTA PLASMA TYPE 2 DIABETICS Objectives: This study examines the composition dependence of essential fatty acids in EDTA plasma of type 2 diabetes on the degree of glycemic expressed by the concentration of glycated hemoglobin (HbA1c). The main objective of this pilot study is to contribute to the development of diabetes diagnosis and expand knowledge of the pathogenesis of this disease. Methods: Lipid extracts from deproteinated plasma samples were separated by preparative thin-layer chromatography into five fractions (phospholipids, diacylglycerols, free fatty acids, triglycerides and cholesterol esters). All fractions were isolated and after the addition of internal standard (cis-13,16,19-docosatrienoic acid, 10 µg / ml) hydrolyzed and converted to corresponding methyl esters of fatty acids. Thus prepared samples were analyzed using a gas chromatograph. Results: 37 anonymized plasma samples randomly selected type 2 diabetic patients were processed in this study. Furthermore, there were used 27 healthy blood donors plasma samples as a control group. For purposes of the study, samples were categorized into three groups according to level of glycosidic hemoglobin (HbA1c). This indicator appears to be most reliable because it is affected by poor conditions (such as non fasting) prior to collection, and highlights the development of blood glucose in the last 4 to 6 weeks. Conclusions: For studied diabetic patients was found to decrease the concentration of essential fatty acids in dependence to the increasing concentration of HbA1c. Besides, it was found to increase in diabetics concentration of saturated fatty acids depending on the increasing concentration of HbA1c. As previously described lipotoxicity saturated fatty acids can be found changes in the plasma concentrations of acids give rise to insulin resistance and the consequential metabolic complications leading to the development of metabolic syndrome followed by type 2 diabetes. diabetes mellitus, essential fatty acids, gas chromatography, thin layer chromatography

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