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CHROMATOGRAPHIC ANALYSIS OF FATTY ACIDS IN HUMAN PLASMA
Authors: Líbalová Martina | Laštovička Petr | Čermák Tomáš | Čegan Alexander
Year: 2014
Type of publication: ostatní - článek ve sborníku
Name of source: List of Abstracts EIDS Summer Workshop 2014
Publisher name: Univerzita Pardubice
Place: Pardubice
Page from-to: 55-55
Titles:
Language Name Abstract Keywords
eng CHROMATOGRAPHIC ANALYSIS OF FATTY ACIDS IN HUMAN PLASMA Obesity is currently a serious health problem. It is expected that these diseases may have a common pathogenesis and multiorgan involvement indicates severe metabolic disorder accompanied by insulin resistance. Given that during the last twenty years could not be found compelling evidence linking the hormonal effect of adipose tissue insulin resistance, but that in obese completely actually exists, it is believed that an important cause of insulin resistance may be an enzymatic abnormality in the metabolism of fatty acids. Lipid extracts from deproteinated plasma samples were separated by preparative thin-layer chromatography into five fractions (phospholipids, diacylglycerols, free fatty acids, triglycerides and cholesterol esters). All fractions were isolated and after the addition of internal standard (cis-13,16,19-docosatrienoic acid, 10 µg / ml) hydrolyzed and converted to corresponding methyl esters of fatty acids. Thus prepared samples were analyzed using a gas chromatograph. 10 anonymized plasma samples randomly selected type 2 diabetic patients were processed in this study. Furthermore, there were used 4 healthy blood donors plasma samples as a control group. For purposes of the study, samples were categorized into three groups according to level of glycosidic hemoglobin (Hb). Lipid metabolism diabetes 2 is only slightly different from the healthy population - the changes are found only in fraction triglyceride and free fatty acids. The TG fraction produced by the liver, reduced concentration of unsaturated fatty acids , mainly oleic and palmitic oil and increased concentration of saturated fatty acids , especially stearic and palmitic. All of this is attributed to the diminished activity of hepatic stearoyl -CoA desaturase. Slightly increased content of saturated fatty acids , mainly palmitic prolonged exposure reduces the production of insulin by the beta cells of pancreas , induces resistance to insulin , and increased hepatic steatosis. diabetes mellitus, lipids, desaturase, elongase, gas chromatography, thin layer chromatography

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