Publication detail

Hydrophilic interaction liquid chromatography of fluorescently labelled oligosaccharides

Authors: Vaňková Nikola | Česla Petr | Fischer Jan

Year: 2014

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Hydrophilic interaction liquid chromatography of fluorescently labelled oligosaccharides	Human body is fulfilled with different types of saccharides (glycans) either in free form or conjugated to proteins or lipids. Conjugated glycans play important role in various biological processes such as cell recongnition, cell-cell interaction, inflammation and disease progression. Glycans could be divided into three basic groups: monosaccharides, oligosaccharides and polysaccharides. Oligosaccharides consist of 2 – 10 monosaccharide units, which are linked by glycosidic bond [1, 2]. The oligosaccharides do not absorb the wavelength above 200 nm. Therefore it is essential to add chromophore or fluorophore group to glycan structure for sensitive detection. Glycans are usually derivatized at their reducing end by reductive amination. In this work, we compared common derivatization reagents used for fluorescent labeling of glycans including 2-aminobenzamide (2-AB), 2-aminobenzoic acid (2-AA), 2-aminopyridine (2-PA) and monopotassium 7-amino-1,3-naphthalenedisulfonate hydrate (ANDS) [1, 3]. The working conditions (columns, gradient profiles, mobile phase flow and additives) of hydrophilic interaction liquid chromatographic separation of labeled glycans were optimized with the aim of coupling to the capillary zone electrophoresis in two dimensional system.

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