Publication detail

Comparison of acetaminophen hepatotoxicity and nephrotoxicity in vitro

Authors: Roušar Tomáš | Vrbová Martina | Kučera Otto | Roušarová Erika | Červinková Zuzana

Year: 2014

Type of publication: ostatní - článek ve sborníku

Name of source: Toxicology Letters

Publisher name: Elsevier Ireland Ltd

Place: Shannon

Page from-to: S81

Titles:

Language	Name	Abstract	Keywords
eng	Comparison of acetaminophen hepatotoxicity and nephrotoxicity in vitro	Acetaminophen (APAP) is a frequently used analgesic and antipyretic. The overdose can cause hepatotoxicity and nephrotoxicity. Acetaminophen toxicity has been considered as the most often cause of acute liver failure and that is why APAP hepatotoxicity has been studied in a number of in vitro studies. On the other hand, acetaminophen nephrotoxicity has been estimated only in some experiments. Therefore, the goal of our study was to compare hepatotoxic and nephrotoxic effect of acetaminophen in vitro. We used cultivated rat hepatocytes to characterize APAP hepatotoxicity. To elucidate the acetaminophen nephrotoxicity, we used human kidney cells (HK-2). Both cell types were incubated with acetaminophen (0-10 mM) for up to 24 h. We measured the LDH leakage, WST-1 to determine the changes in viability. Glutathione content and ROS production was measured for detection of oxidative stress.	Acetaminophen; mitochondria; toxicity; glutathione; ROS

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