Publication detail

INFLUENCE OF CHOSEN MICROBES AND SOME CHEMICAL SUBSTANCES ON THE PRODUCTION OF AFLATOXINS

Year: 2015

Type of publication: článek v odborném periodiku

Name of source: Potravinarstvo

Publisher name: HACCP Consulting

Place: Nitrianske Hrnčiarovce

Page from-to: 9-17

Titles:

Language	Name	Abstract	Keywords
cze	Vliv vybraných mikrobů a některých chemických látek na produkci aflatoxinů	Cílem této práce bylo zjištění produkce aflatoxinů metodou HPLC a zjišťování faktorů ovlivňujících jejich tvorbu.	aflatoxiny; konzervanty; Pythium oligandrum; Rhodotorula; inhibice; Aspergillus flavus; A. parasiticus
eng	INFLUENCE OF CHOSEN MICROBES AND SOME CHEMICAL SUBSTANCES ON THE PRODUCTION OF AFLATOXINS	The aim of this work was the detection of aflatoxins by the HPLC method and the ascertainment of factors influencing their production. A. parasiticus CCM F-108, A. parasiticus CCF 141, A. parasiticus CCF 3137 and two isolates A. flavus were used. These toxigenic isolates were recovered from spice (strain 1) and wraps (strain 2). The gene for the production of aflatoxin B1 for each species of fungi was detected using an optimized PCR method. Rhodotorula spp., Lactococcus lactis subsp. lactis CCM 1881, Flavobacterium spp. and fungal strain Pythium oligandrum were tested for inhibition of aflatoxins production and fungal growth. Having used the HPLC detection, various preservatives (propionic acid, citric acid, potassium sorbate) were tested from the viewpoint of their influence on the growth of aflatoxigenic fungi followed by the production of aflatoxins. The growth of A. flavus and A. parasiticus and aflatoxin production in Potato Dextrose Agar supplemented with propionic acid (1000-2000-3000 mg/kg), citric acid (2000-3000-4000 mg/kg) and potassium sorbate (500-800-1000 mg/kg) was tested by Agar Dilution Method. After 72 h of incubation was evaluated growth of fungi, all samples were frozen for later extraction and aflatoxins quantification by HPLC. Effect of peptone and sucrose additions were studied in yeast extract (2%) supplemented with peptone (5-10-15%) or sucrose (15%). Growth inhibition of Aspergillus by Pythium oligandrum was tested on wood surface. As shown, the highest inhibition effect on the aflatoxins production was obtained when propionic acid was applied in concentrations since 1000 mg/kg. A total inhibition of the fungi growth and aflatoxins production was observed in all samples containing peptone in the concentration range tested. Significant limitation of the growth and production of aflatoxins was also observed in the presence of other microorganisms such like Pythium oligandrum and Rhodotorula spp.	aflatoxin; preservatives; Pythium oligandrum; Rhodotorula; inhibition; Aspergillus flavus; A. parasiticus

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