Publication detail

Fluorescence resonance energy transfer of gas-phase ions under ultra high vacuum and ambient conditions

Year: 2014

Type of publication: článek v odborném periodiku

Name of source: Physical Chemistry Chemical Physics

Publisher name: Royal Society of Chemistry

Place: Cambridge

Page from-to: 8911-8920

Titles:

Language	Name	Abstract	Keywords
cze	Fluorescenční rezonanční přenos energie plynných iontů za ultra vysokého vakua	Práce se zabývá fluorescenčním rezonančním přenosem energie plynných iontů za ultra vysokého vakua a okolních podmínek.
eng	Fluorescence resonance energy transfer of gas-phase ions under ultra high vacuum and ambient conditions	We report evidence for fluorescence resonance energy transfer (FRET) of gas-phase ions under ultra high vacuum conditions (10(-9) mbar) inside a mass spectrometer as well as under ambient conditions inside an electrospray plume. Two different FRET pairs based on carboxyrhodamine 6G (donor) and ATTO590 or Bodipy TR (acceptor) dyes were examined and their gas-phase optical properties were studied. Our measurements indicate a different behavior for the two FRET pairs, which can be attributed to their different conformations in the gas phase. Upon desolvation via electrospray ionization, one of the FRET pairs undergoes a conformational change that leads to disappearance of FRET. This study shows the promise of FRET to obtain a direct correlation between solution and gas-phase structures.	ELECTRON-TRANSFER; MOLECULAR-DYNAMICS; CONFORMATIONAL DYNAMICS; FORCE-FIELD; PROBE; TRYPTOPHAN; SPECTROSCOPY; DYES

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