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Comparison of Mass Spectrometry Based Approaches in Lipidomics
Authors: Holčapek Michal | Cífková Eva | Lísa Miroslav | Jirásko Robert | Chagovets Vitaliy Viktorovich
Year: 2014
Type of publication: ostatní do riv
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
cze Comparison of Mass Spectrometry Based Approaches in Lipidomics Lipidomics deals with the complex lipidomic characterization of dynamic biological systems. The ion suppression effects could reduce the sensitivity for determination of trace lipids, but on the other hand the advantage is that the sample is infused at the constant eluent composition and flow rate, so the suppression effects can be compensated by internal standards and the quantitative data are reliable, as demonstrated in numerous large-scale clinical studies. Matrix-assisted laser desorption/ionization (MALDI)-MS is another techniques, which is often used without a chromatographic separation, but the well-known limitation of MALDI for the quantitative purposes is less signal stability and reproducibility depending on the matrix used and the sample preparation. The systematic optimization of matrix selection and deposition approaches (spraying, electrospraying and sublimation) or matrix-free techniques on dedicated metal plates shows a potential of MALDI/LDI for the (semi-)quantitative lipidomic analysis. We will present examples of individual separation modes suitable for distinguishing of particular type of isomerism, such as reversed-phase, silver-ion or chiral chromatography used either in conventional HPLC mode or preferably under UHPLC conditions using sub-2 μm particles and high back pressures or alternatively core-shell sub-3 μm particles. Recently, the potential of supercritical fluid chromatography (SFC) has been realized with a great separation efficiency and very short analysis times compared to LC/MS. Various quantitative LC/MS and SFC/MS approaches will be discussed as well. Ion mobility used in configuration with mass spectrometry can bring supplementary separation of lipids from background ions and separation of lipid classes and species inside classes. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic).
eng Comparison of Mass Spectrometry Based Approaches in Lipidomics Lipidomics deals with the complex lipidomic characterization of dynamic biological systems. The ion suppression effects could reduce the sensitivity for determination of trace lipids, but on the other hand the advantage is that the sample is infused at the constant eluent composition and flow rate, so the suppression effects can be compensated by internal standards and the quantitative data are reliable, as demonstrated in numerous large-scale clinical studies. Matrix-assisted laser desorption/ionization (MALDI)-MS is another techniques, which is often used without a chromatographic separation, but the well-known limitation of MALDI for the quantitative purposes is less signal stability and reproducibility depending on the matrix used and the sample preparation. The systematic optimization of matrix selection and deposition approaches (spraying, electrospraying and sublimation) or matrix-free techniques on dedicated metal plates shows a potential of MALDI/LDI for the (semi-)quantitative lipidomic analysis. We will present examples of individual separation modes suitable for distinguishing of particular type of isomerism, such as reversed-phase, silver-ion or chiral chromatography used either in conventional HPLC mode or preferably under UHPLC conditions using sub-2 μm particles and high back pressures or alternatively core-shell sub-3 μm particles. Recently, the potential of supercritical fluid chromatography (SFC) has been realized with a great separation efficiency and very short analysis times compared to LC/MS. Various quantitative LC/MS and SFC/MS approaches will be discussed as well. Ion mobility used in configuration with mass spectrometry can bring supplementary separation of lipids from background ions and separation of lipid classes and species inside classes. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic). Mass Spectrometry; Lipidomics

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