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LC/MS and SFC/MS in Lipidomic Analysis

Authors: Holčapek Michal | Cífková Eva | Lísa Miroslav

Year: 2014

Type of publication: ostatní do riv

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
cze	LC/MS and SFC/MS in Lipidomic Analysis	Lipidomic analysis is challenging due to enormous complexity of lipid structures. The mass spectrometry (MS) plays a key role in the quantitative lipidomics either without separation (shotgun ESI-MS or MALDI-TOF) or in separation coupling with MS (mainly LC/MS and SFC/MS). Each modality can bring complementary information, which can be integrated in one overall methodology. Various quantitative LC/MS and SFC/MS approaches will be discussed as well. The goal of this work is the development of complementary nontargeted and targeted MS based method for the comprehensive characterization of biological samples, which is applied for the characterization of changes caused by the tumor growth in comparison with surrounding healthy tissues.
eng	LC/MS and SFC/MS in Lipidomic Analysis	Lipidomic analysis is challenging due to enormous complexity of lipid structures. The mass spectrometry (MS) plays a key role in the quantitative lipidomics either without separation (shotgun ESI-MS or MALDI-TOF) or in separation coupling with MS (mainly LC/MS and SFC/MS). Each modality can bring complementary information, which can be integrated in one overall methodology. Various quantitative LC/MS and SFC/MS approaches will be discussed as well. The goal of this work is the development of complementary nontargeted and targeted MS based method for the comprehensive characterization of biological samples, which is applied for the characterization of changes caused by the tumor growth in comparison with surrounding healthy tissues.	LC/MS; SFC/MS; Lipidomic Analysis