Publication detail

Effects of fatty acyl chain length, double-bond number and matrix on phosphatidylcholine responses in matrix-assisted laser desorption/ionization on an Orbitrap mass spectrometer

Authors: Chagovets Vitaliy Viktorovich | Lísa Miroslav | Holčapek Michal

Year: 2015

Type of publication: článek v odborném periodiku

Name of source: Rapid Communication in Mass Spectrometry

Page from-to: 2374-2384

Titles:

Language	Name	Abstract	Keywords
cze	Vliv délky acylového řetězce, počtu dvojných vazeb a matrice na odezvu fosfatidylcholinu při použití hmotnostní spektrometrie s ionizací desorpcí laserem za účasti matrice	Ionizace desorpcí laserem za účasti matrice (MALDI-MS) bylo použito pro rychlou kvantitativní analýzu fosfatidylcholinu (PC). V této práci byl studován vliv matric: 2,5-dihydroxybenzoová kyselina (DHB), 1,5-diamino naftalen a 9-aminoakridin (9AA), délky mastného acylového řetězce, nasycenosti na odezvu PC.
eng	Effects of fatty acyl chain length, double-bond number and matrix on phosphatidylcholine responses in matrix-assisted laser desorption/ionization on an Orbitrap mass spectrometer	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is used for the fast qualitative and quantitative analysis of phosphatidylcholines (PC). Fatty acyl chain lengths and the number of double bonds (DB) affect relative responses of PC; hence the determination of correction factors of individual PC is important for the accurate quantitation. The signal intensity in MALDI-MS strongly depends on the matrix; therefore, the following matrices typically used in lipidomics are studied in the present work: 2,5-dihydroxybenzoic acid (DHB), 1,5- diaminonaphthalene (DAN) and 9-aminoacridine (9AA).	DHB; PC; MALDI; DB

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