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Long-term stability of cancer biomarkers in human serum: biomarkers of oxidative stress and redox status, homocysteine, CRP and the enzymes ALT and GGT
Authors: Jansen Eugene | Beekhof Piet | Viezeliene Dale | Mužáková Vladimíra | Skalicky Jiri
Year: 2015
Type of publication: článek v odborném periodiku
Name of source: Biomarkers in Medicine
Publisher name: Future Medicine Ltd
Place: LONDON
Page from-to: 425-432
Titles:
Language Name Abstract Keywords
cze Dlouhodobá stabilita rakovinných biomarkerů v lidském séru: biomarkery oxidačního stresu a redoxního stavu, homocystein, CRP a enzymy ALT a GMT Pět biomarkerů často užívaných ve studiích výzkumu rakoviny a epidemiologických studiích bylo testováno pro jejich stabilitu po dobu 12 měsíců skladování séra při -20 a -70 stupňů C. Dlouhodobá stabilita; biomarkery; gama-glutamyltransferáza; alanin aminotransferáza; homocystein; CRP; hydroperoxidy; stabilita; skladování
eng Long-term stability of cancer biomarkers in human serum: biomarkers of oxidative stress and redox status, homocysteine, CRP and the enzymes ALT and GGT Aim: Five frequently used biomarkers in cancer research and epidemiological studies were tested for their assay stability upon storage of serum for 12 months at -20 and -70/-80 degrees C. Materials & methods: The biomarker assays include reactive oxygen metabolites (ROM), the total thiol levels (TTL), homocysteine (HCy), C-reactive protein (HS-CRP) and two liver enzymes, alanine aminotransferase (ALT) and -glutamyltransferase (GGT). Results: The assays for ROM, HCy, HS-CRP and GGT were stable in human serum samples at the two temperatures tested. The two other assays TTL and ALT, however, showed statistically significant differences in their stability between -20 and -80 degrees C. Conclusion: Therefore, storage at -80 degrees C is advised to maintain a reliable assay outcome when serum samples have to be stored for longer periods. gamma-glutamyltransferase; alanine aminotransferase; cancer biomarkers; C-reactive protein; homocysteine; hydroperoxides; stability; storage; total thiols

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