Publication detail

Characterization and in vitro evaluation of the behaviour of 6-carboxycellulose/Ag(I) in the presence of microorganisms

Year: 2015

Type of publication: článek v odborném periodiku

Name of source: Cellulose Chemistry and Technology

Publisher name: Editura Academiei Romane

Place: Bukurešť

Page from-to: 291-301

Titles:

Language	Name	Abstract	Keywords
cze	Charakterizace 6-karboxycelulózy modifikované stříbrnou solí a studium jejího chování in vitro v přítomnosti vybraných mikroorganizmů	Za využití vybraných analytických metod (C-13 CP/MAS NMR, FAAS, XRD, SEC) byla provedena charakterizace nového typu antimikrobiálního materiálu složeného z oxidované celulózy modifikované stříbrnou solí. Provedena byla dále studie chování uvedeného typu materiálu in vitro v přítomnosti několika patogenů (Aspergillus fumigatus, Candida albicans, Pseudomonas aeruginosa a Staphylococcus epidermidis) a identifikace případných strukturálních změn . Pro tento účel byly aplikovány metody FAAS, FTIR, ICP-oa-TOF-MS, HPLC, SEC a SEM.	oxidovaná celulóza; degradace; antimikrobiální materiály; analýza polymerů; stříbro
eng	Characterization and in vitro evaluation of the behaviour of 6-carboxycellulose/Ag(I) in the presence of microorganisms	A new type of an antimicrobial material based on silver salt (chloride) adsorbed onto 6-carboxycellulose (OC/Ag(I)) was characterized using solid-state carbon-13 cross-polarization magic angle spinning nuclear magnetic resonance (C-13 CP/MAS NMR) spectroscopy, flame atomic absorption spectrometry (FAAS), X-ray diffraction (XRD) and size exclusion chromatography (SEC). In vitro investigations of the behaviour of the material were carried out in buffered nutrient media (pH 7.0 or 5.4) in the presence of several human pathogens, such as Aspergillus fumigatus, Candida albicans, Pseudomonas aeruginosa and Staphylococcus epidermidis at 37 degrees C within a period of one week. The concentration of Ag released into the liquid nutrient media was measured by FAAS. Further, the composition of the resulting liquid and solid phase was characterized by different chromatographic methods, Fourier transform infrared spectroscopy (FTIR), FAAS, and inductively coupled plasma orthogonal acceleration time-of-flight mass spectrometry (ICP-oa-TOF-MS) and scanning electron microscopy (SEM). The results showed that none of the investigated microorganisms have shown significant degradation potential of OC structure. Only a partial release of Ag was observed, compared to the initial samples, regardless of the presence of the microorganism strain and both investigated pH values. Other noticeable changes observed in the molecular structure of OC were due to the increasing neutralization degree upon cationization by the ions present in the nutrient media.	oxidized cellulose; silver salt of oxidized cellulose; degradation; antimicrobial materials; analysis of polymer; silver

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