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Simultaneous measurement of malondialdehyde, formaldehyde, acetaldehyde, and propionaldehyde to monitor the oxidative stress in humans
Authors: Kanďár Roman | Drábková Petra | Richter Michal | Rusnoková Marcela | Čegan Alexander
Year: 2015
Type of publication: článek v odborném periodiku
Name of source: Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
Page from-to: 45-56
Titles:
Language Name Abstract Keywords
cze Simultánní stanovení malondialdehydu, formaldehydu, acetaldehydu a propionaldehydu pro monitorování oxidačního stresu u lidí Je zde popsána metoda pro stanovení malondialdehydu, formaldehydu, acetaldehydu a propionaldehydu v lidské plazmě. Vzorky plazmy byly získány od dobrovolných dárců krve. Po precipitaci proteinů, byly vzorky derivatizovány 2,4-dinitrofenylhydrazinem a opakovaně extrahovány pentanem. Spojené pentanové extrakty byly v atmosféře dusíku odpařeny do sucha a odparek byl rozpuštěn v acetonitrilu. Dvacet mikrolitrů bylo dávkováno na kolonu LichroCART 125-4, Purospher STAR RP-18e, 5 μm. Deriváty byly separovány gradientovou elucí, mobilní fáze obsahovala acetonitril, vodu a kyselinu octovou. Analytické parametry metody byly vyhovující, variační koeficienty přesnosti v sérii byly pod 12 %, výtěžnosti byly 98,3 % (CV 1,6 %) pro malondialdehyd, 104,4 % (CV 4,0 %) pro formaldehyd, 96,9 % (CV 3,0 %) pro acetaldehyd a 105,9 % (CV 1,7 %) pro propionaldehyd. malondialdehyd; formaldehyd; acetaldehyd; propionaldehyd; oxidační stres; kapalinová chromatografie
eng Simultaneous measurement of malondialdehyde, formaldehyde, acetaldehyde, and propionaldehyde to monitor the oxidative stress in humans Herein, a method is described for the determination of malondialdehyde, formaldehyde, acetaldehyde, and propionaldehyde in human plasma. Plasma samples were obtained from blood donors. After protein precipitation, the samples were derivatized with 2,4-dinitrophenylhydrazine and repeatedly extracted with pentane. So combined pentane extracts were evaporated to dryness, under nitrogen atmosphere, and the dried residue was re-suspended in acetonitrile. Aliquots of 20 μl in acetonitrile were injected onto a LichroCART 125-4, Purospher STAR RP-18e, 5 μm column. The derivatives were analysed with the aid of gradient elution, when using a mobile phase containing acetonitrile, water and acetic acid. The analytical performance of this method has been found to be satisfactory characterised by the intra-assay coefficients of variation were below 12 %. and quantitative recoveries as follows: 98.3% (CV 1.6%) for malondialdehyde, 104.4% (CV 4.0%) for formaldehyde, 96.9% (CV 3.0%) for acetaldehyde, and 105.9% (CV 1.7%) for propionaldehyde. malondialdehyde; formaldehyde; acetaldehyde; propionaldehyde; oxidative stress; liquid chromatography

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