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Assessment of silver nanoparticle toxicity for common carp (Cyprinus carpio) fish embryos using a novel method controlling the agglomeration in the aquatic media
Authors: Opršal Jakub | Blaha Ludek | Pouzar Miloslav | Knotek Petr | Vlček Milan | Hrdá Kateřina
Year: 2015
Type of publication: článek v odborném periodiku
Name of source: Environmental Science and Pollution Research
Publisher name: Springer
Place: Heidelberg
Page from-to: 19124-19132
Titles:
Language Name Abstract Keywords
cze Hodnocení toxicity nanočástic Ag na Kapru obecném (Cyprinus carpio) s využitím nové metody řízení velikosti aglomerátů v kapalném médiu Tvorba aglomerátů a jejich rychlá sedimentace v kapalném médiu má významný vliv na průběh testů toxicity nanomateriálů prováděných na vodních organismech. Předložená práce využívá originální proces řízení velikosti aglomerátů pomocí periodické výměny kapalného média. Frekvence výměny média pro zvolenou maximální velikost aglomerátů 200 a 400 nm je vypočtena pomocí verifikovaného matematického modelu. Výsledky testů s proměnnou frekvencí výměny média byly porovnámy s výsledky klasického semistatického testu. Nanostříbro;aglomerace;rybí embryo;kapr obecný;distribuce velikosti částic
eng Assessment of silver nanoparticle toxicity for common carp (Cyprinus carpio) fish embryos using a novel method controlling the agglomeration in the aquatic media Formation of agglomerates and their rapid sedimentation during aquatic ecotoxicity testing of nanoparticles is a major issue with a crucial influence on the risk assessment of nanomaterials. The present work is aimed at developing and testing a new approach based on the periodic replacement of liquid media during an ecotoxicological experiment which enabled the efficient monitoring of exposure conditions. A verified mathematical model predicted the frequencies of media exchanges which checked for formation of agglomerates from silver nanoparticles AgNP with 50 nm average size of the original colloid. In the model experiments, embryos of common carp Cyprinus carpio were exposed repeatedly for 6 h to AgNPs (5-50 mu m Ag L-1) either under semistatic conditions (exchange of media after 6 h) or in variants with frequent media exchanges (varying from 20 to 300 min depending on the AgNP colloid concentration and the desired maximum agglomerate size of 200 or 400 nm). In contrast to other studies, where dissolved free metals are usually responsible for toxic effects, our 144-h experiments demonstrated the importance of AgNP agglomerates in the adverse effects of nanosilver. Direct adsorption of agglomerates on fish embryos locally increased Ag concentrations which resulted in pronounced toxicity particularly in variants with larger 400 nm agglomerates. The present study demonstrates the suitability of the novel methodology in controlling the conditions during aquatic nanomaterial toxicity testing. It further provided insights into the mechanisms underlying the effects of AgNP, which rank on a global scale among the most widely used nanomaterials. Nanosilver;Agglomeration;Fish embryo;Cyprinus carpio;Particle size distribution

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