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Two Temperature Sorption Strategy for Screening of Volatile Compounds in Barley and Wheat Malts by Method Based on Solid Phase Microextraction
Authors: Adam Martin | Eisner Aleš | Adámková Karolína | Bajerová Petra | Ventura Karel
Year: 2015
Type of publication: ostatní - článek ve sborníku
Name of source: 9th International Conference on Instrumental Methods of Analysis: Modern Trends and Applications: Book of Abstracts
Publisher name: Technological Educational Institute of Peloponnese
Place: Kalamata
Page from-to: 193
Titles:
Language Name Abstract Keywords
eng Two Temperature Sorption Strategy for Screening of Volatile Compounds in Barley and Wheat Malts by Method Based on Solid Phase Microextraction In this study, a modern sampling approach based on the use of two extraction temperatures in the same headspace solid-phase microextraction (HS-SPME) procedure was optimised and evaluated for the analysis of aromaprofile of barley and wheat malt samples. Separation and identification of target compounds was realised by gas chromatography–mass spectrometry (GC–MS) method. This modern sorption strategy is aiming at extracting a significantly higher number of compounds with wider range of volatilities comparing to conventions sorption strategy using only one sorption temperature. As an analytical tool, experimental designs were used for the method optimisation. First of all, the most significant factors affecting the whole procedure were evaluated by the approach based on the Plackett–Burman design. After that, the final experimental conditions were established by five-level orthogonal central composite design optimisation approach followed by response surfaces modelling. The proposed method presents a very good compromise of conditions under which the right combination of sorption temperatures could achieve a much better extraction enabling identification of more volatile and semi-volatile compounds in obtained chromatograms. This method was compared to conventional approach based on the sorption at a single extraction temperature. It was confirmed that the presented method provides a better results considering the response in terms of both the total peak areas and mainly the number of identified compounds. The use of two extraction temperatures in a single assay for the HS-SPME procedure proved to be a very good alternative for the screening of compounds present in barley and wheat malts with a wide range of volatilities. SPME; GC-MS; Aromaprofile; Malts

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