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Evaluation of Solid Phase Microextraction Method Using Two Temperature Sorption Approach for Aromaprofile Analysis of Barley and Wheat Malts
Authors: Adam Martin | Adámková Karolína | Eisner Aleš | Ventura Karel
Year: 2015
Type of publication: ostatní - článek ve sborníku
Name of source: 7th International Symposium on RECENT ADVANCES IN FOOD ANALYSIS: Book of Abstracts
Publisher name: Vysoká škola chemicko-technologická v Praze
Place: Praha
Page from-to: 239
Titles:
Language Name Abstract Keywords
eng Evaluation of Solid Phase Microextraction Method Using Two Temperature Sorption Approach for Aromaprofile Analysis of Barley and Wheat Malts The main aim of this study was the evaluation of a modern sampling approach based on the use of two sorption temperatures in the one headspace solid-phase microextraction (HS–SPME) procedure and its applicability to the analysis of aromaprofile of barley and wheat malt samples. For separation and identification of target compounds the method of gas chromatography–mass spectrometry (GC–MS) together with comparison of retention indices of individual compounds with those found in spectral libraries and/or available on-line databases were used. This modern sorption strategy based on using more sorption temperatures in one procedure, is aiming at extracting a significantly higher number of compounds with wider range of volatilities comparing to conventions sorption strategy using only one sorption temperature. As an analytical tool, experimental designs were used for the method optimisation. First of all, the most significant factors affecting the whole procedure were evaluated by the Plackett–Burman design. After that, the final experimental conditions were established by five-level orthogonal central composite design followed by response surface modelling. During the optimisation experiments it was observed that higher temperature improves the analyte response in the case of less volatile compounds whereas lower temperature is more advantageous in the case of more volatile analytes. The proposed method presents a very good compromise of conditions under which the right combination of sorption temperatures could achieve a much better extraction enabling identification of more volatile and semi-volatile compounds in obtained chromatograms. The proposed method was compared to conventional HS–SPME using single temperature sorption approach. It was confirmed that the presented method provides a better results considering the response in terms of both the total peak areas and mainly the number of identified compounds. Malts; SPME; GC-MS; Aromaprofile

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