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Arcobacter spp. – modern method for detection
Authors: Šilha David | Šilhová Lucie | Vytřasová Jarmila
Year: 2015
Type of publication: kapitola v odborné knize
Name of source: Laboratory Techniques with Applicability in Medical Practise
Publisher name: LAP Lambert Academic Publishing AG & Co. KG
Place: Saarbrücken
Page from-to: 33-45
Titles:
Language Name Abstract Keywords
cze Arcobacter spp. – moderní metody detekce Tato kapitola je zaměřena na detekování biofilmových a bakteriálních buněk bakterií rodu Arcobacter a Campylobacter. Jedná se o bakterie, které mohou způsobovat mnoho onemocnění. Zejména v posledních letech jsou arkobaktery velice často isolovány z klinických vzorků zvířat, potravin živočišného původu a různých typů vod. Existuje několik moderních metodik pro jejich detekci. Arcobacter spp.; FISH; PCR; EMA/PMA-PCR; detekce patogenů
eng Arcobacter spp. – modern method for detection This chapter focuses on detecting biofilm and planktonic bacterial cells of the genera Arcobacter and Campylobacter. These bacteria can cause a lot of diseases. Especially, in the last decade, arcobacters have been increasingly isolated from feces of clinically healthy and ill animals, foods of animal origin and various types of water. There are several modern methods for bacteria detection which can be suitable tool. A multiplex-polymerase chain reaction (PCR) method was introduced and optimized for detecting biofilm while using the intercalating dyes ethidium monoazide (EMA) and propidium monoazide (PMA) for collection strains of bacteria of the genus Arcobacter with subsequent application on samples of prepared biofilms. Fluorescence in situ hybridization was used to detect biofilm and planktonic cells of selected microorganisms. This method was optimized and subsequently applied to biofilm samples prepared on stainless steel coupons. The study results indicate that fluorescence in situ hybridization is suitable for detecting biofilm and planktonic cells of the studied bacteria. Arcobacter spp.; FISH; PCR; EMA/PMA-PCR; pathogen detection

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