Publication detail

On-line mutidimensional liquid column chromatography

Authors: Jandera Pavel | Hájek Tomáš | Staňková Magda | Česla Petr | Škeříková Veronika

Year: 2015

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	On-line mutidimensional liquid column chromatography	The main objective of multidimensional (MD) chromatography is increasing the number of resolved compounds in a complex sample (peak capacity of the separation system). While off-line MD by TLC, GC and CLC techniques have been used for long time, on-line comprehensive technique where the whole sample is subject to separation in each dimension are relatively recent, especially as HPLC separations are concerned. The main issue in on-line two-dimensional (2D) separations is the necessity of accomplishing the second-dimension separation of the fraction collected from the first-dimension column in the short time (1-2 min or even less) available for the collection of the next fraction. For maximum peak capacity, different separation mechanisms should be used in each dimension of an MD system (RP, HILIC, Ion Exchange), however the mobile phases in each system should be compatible. For fast 2D separations, it is advantageous using gradient elution simultaneously in each dimension. The choice of optimum column combinations, dimensions and separation conditions (stationary and mobile phases, flow rate, fraction cycle time, etc.,) are discussed and examples of practical applications of on-line MD techniques are shown.

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