Publication detail

Monolithic and Core-shell columns in fast uni-, two- and three-dimensional HPLC separations of phenolic compounds

Authors: Jandera Pavel | Hájek Tomáš | Staňková Magda

Year: 2015

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Monolithic and Core-shell columns in fast uni-, two- and three-dimensional HPLC separations of phenolic compounds	UHPLC on sub-2 micron particle columns is a straightforward way to increasing the speed of HPLC separation, at the cost of very high pressure and special instrumentation. However, fast efficient separation are possible also on short core-shell of monolithic columns and often enable fast (1 min or less) and efficient separation at pressures which can be achieved with conventional HPLC instrumentation, due to low flow resistance. This feature is especially attractive for fast separation in the second dimension of comprehensive two-dimensional HPLC, where the analysis of a fraction collected from the first dimension must be performed on-line in the second dimension within the short time available for the collection of the next fraction. Short core-shell or silica-based monolithic columns in the second dimension can be combined with a longer microbore or capillary column in the first dimension. For this purpose, we developed an efficient organic polymer zwitterionic column, which can be used either in reversed-phase or in normal-phase (HILIC) mode for efficient separations of phenolic acids, polyphenolic compounds, flavonoids and related compounds.

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