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Towards multifunctional monolithic capillary device: sample focusing, separation, and electrochemical detection of neurotransmitters in one single capillary
Authors: Urban Jiří | Metelka Radovan | Janků Simona
Year: 2015
Type of publication: ostatní - článek ve sborníku
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
cze Příprava multifunkční monolitické kapilární kolony Pomocí 4-vinylfenyl borité kyseliny jsme připravili monolitické stacionární fáze, které jsme použili k selektivnímu záchytu nervových přenašečů obsahujících cis-diol funkční skupinu. Optimalizovali jsme přípravu monolitických fází a jejich spojení s kapalinovou chromatografií. Využili jsme povrchovou úpravu monolitu k přípravě kolon integrujících dvě funkce, selektivní záchyt a separaci. Integrací uhlíkových a stříbrných vláken uvnitř kapiláry jsme připravili elektrochemický detektor uvnitř křemenné kapiláry. Detektor poskytoval lineární odezvu přes čtyři řády. kapilární kolony; polymerní monolity, nervové přenašeče, integrovaná kolona,
eng Towards multifunctional monolithic capillary device: sample focusing, separation, and electrochemical detection of neurotransmitters in one single capillary We have used 4-vinylphenylboronic acid monomer to prepare monolithic stationary phase that selectively entraps neurotransmitters on the basis of cis-diol functionality. We have optimized its online coupling with a continuous flow of the sample with the emphasis on the effect of sample flow-rate and switching time on the recovery of neurotransmitters. In the next step, we have tested several approaches how to prepare monolithic capillary column which integrates sample focusing with separation. This included coupling of columns with a zero volume union, copolymerization of polymerization mixtures, and surface-initiated grafting. To incorporate electrochemical detection at the end of monolithic capillary column, we have optimized experimental conditions used to fix carbon microfibers by a monolithic stationary phase formed at the end of the capillary. In parallel, we have tested an outer fixation of a tetrode inside the capillary. The application of a tetrode is advantageous since it could provide two-electrode system by interconnecting of electrodes to operate in biamperometry mode, when there is no need to use laborious and potentially unstable reference electrode of small dimensions. Linear dependence of peak heights on the concentration of catecholamines was observed in the concentration range of 5×10−4 – 1×10−5 M. Presented work contributes significantly to two main areas of current research: (i) miniaturization of liquid chromatography-based instrumentation and (ii) an online hyphenation of sample focusing, HPLC separation, and electrochemical detection in one device. capillary column; electrochemical detection; integrated device; neurotransmitters; polymer monoliths;

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