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Multifunctional monolithic capillary column with incorporated sample focusing, separation, and electrochemical detection
Authors: Urban Jiří | Metelka Radovan | Janků Simona | Komendová Martina
Year: 2015
Type of publication: ostatní - článek ve sborníku
Name of source: 21th International Symposium on Separation Sciences, ISSS 2015: Book of Abstracts
Publisher name: National Institute of Chemistry
Place: Ljubljana
Page from-to: 11
Titles:
Language Name Abstract Keywords
cze Vícefunkční monolitická kapilární kolona Připravili jsme vícefunkční monolitickou kapilární kolonu. Pomocí povrchové modifikace technikou roubování jsme připravili stacionární fázi poskytující jak selektivní zachycení vzorku nervových přenašečů, tak i účinnou separaci těchto malých molekul. Fixací uhlíkového a stříbrného vlákna uvnitř kapiláry jsme připravili elektrochemický detektor poskytující lineární odezvu a limit detekce 110 pg dopaminu. polymerní monolity, kapilární kolony, nervové přenašeče, integrovaná kolona
eng Multifunctional monolithic capillary column with incorporated sample focusing, separation, and electrochemical detection One of the main advantages of organic polymer-based monoliths is their simple and straightforward preparation and subsequent surface modification. In this work, we describe our recent attempts in the development of a single monolithic capillary column that offers integrated sample focusing, separation, and electrochemical detection of monoamine neurotransmitters. Fast, simple, and efficient quantification of neurotransmitters plays a crucial role in a proper diagnosis and treatment control of several neurological diseases. At first, we have used 4-vinylphenylboronic acid monomer to prepare monolithic stationary phase that selectively entraps neurotransmitters on the basis of cis-diol functionality. We have optimized its online coupling with another monolithic capillary column providing neurotransmitters separation. In the next step, we have tested several approaches how to prepare monolithic capillary column which integrates sample focusing with separation. This included coupling of columns with a zero volume union, copolymerization of polymerization mixtures, and surface-initiated grafting. To incorporate electrochemical detection at the end of monolithic capillary column, we have optimized experimental conditions used to fix carbon microfibers (7 m diameter) by a monolithic stationary phase formed at the end of the capillary. In parallel, we have tested an outer fixation of a tetrode fiber (with an outer diameter of 100 m) consisting of four platinum/tungsten alloy electrodes isolated by quartz glass. The application of a tetrode is advantageous since it could provide two-electrode system by interconnecting of electrodes to operate in biamperometry mode, when there is no need to use laborious and potentially unstable reference electrode of small dimensions. Linear dependence of peak heights on the concentration of catecholamines was observed in the concentration range of 5×10−4 – 1×10−5 M. polymer monolith; integrated device; neurotransmitters;

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