Login for students

Login for employees

Publication detail

Peak Capacity in Two-dimensional Separations Combining Liquid Chromatography with Capillary Electrophoresis
Authors: Vaňková Nikola | Váňová Jana | Nováková Zuzana | Křenková Jana | Fischer Jan | Česla Petr
Year: 2015
Type of publication: ostatní - článek ve sborníku
Name of source: 22nd International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2015): book of abstracts
Publisher name: Finish Society for Chromatography and Mass Spectrometry
Place: Helsinky
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Peak Capacity in Two-dimensional Separations Combining Liquid Chromatography with Capillary Electrophoresis The main purpose for application of two-dimensional separations is the increased peak capacity comparing to the unidimensional systems, which is especially needed for analysis of samples of biological origin. The highest increase of peak capacity with respect to the uni-dimensional separation is achieved for orthogonal separation mechanisms, i.e. for the combination of liquid chromatography with capillary zone electrophoresis. In present work, we have investigated the effects of separation conditions on peak capacity and peak production rate of two-dimensional separation utilizing combination of hydrophilic interaction liquid chromatography with capillary zone electrophoresis for separation of maltooligosaccharides and glycans. We have applied several retention models describing the partitioning of the analytes between the bulk mobile phase and adsorbed water-rich layer, polar and ionic interactions with various stationary phases including silica gel, aminopropyl-, carbamoyl- and zwitterionic sulfobetain-bonded silica gel. The fast electrophoretic separations utilizing conditions compatible with mass spectrometric detection (volatile background electrolytes and short separation capillaries) were used in the second-dimension separations. Finally, the theoretically obtainable peak capacities determined using retention/migration models for developed two-dimensional system were compared and discussed with respect to the undersampling effect observed due to the application of microfluidic flow-gating interface connecting both separation techniques.

Departments

Other Parts

Map

en.mapy.cz

Services

Popular Links

© 2023 University of Pardubice, Studentská 95, 532 10 Pardubice 2
Phone: +420 466 036 111, 466 036 112, 466 036 113
Webmaster, RSS
Data box ID: f5vj9hu
Accessibility Statement
CC BY-NC-ND 4.0Creative CommonsAttributionNonCommercialNoDerivatives