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Optimization of Chiral Separations of Benzthiazole Derivatives of Amino Acids in Capillary Zone Electrophoresis
Authors: Nováková Zuzana | Česla Petr | Fischer Jan | Pejchal Vladimír
Year: 2015
Type of publication: ostatní - článek ve sborníku
Name of source: 10th Balaton Symposium on High-Performance Separation Methods: Book of Abstracts.
Publisher name: Sciences
Place: Veszprém
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Optimization of Chiral Separations of Benzthiazole Derivatives of Amino Acids in Capillary Zone Electrophoresis In recent years, the increased interest in separation of chiral compounds is growing especially in pharmaceutical industry. Enantiomers have the same physicochemical properties in an achiral environment, but possess rapidly different pharmaceutical, toxicological and biological properties1. One of the best techniques for separation of enantiomers containing ionizable functional groups is capillary zone electrophoresis in background electrolytes with addition of chiral selectors. Such selectors form diastereomeric complexes with optical izomers, whose migrate at different velocity with respect to the uncomplexed enatiomers. In this work, the separation of benzothiazole derivatives of amino acids was optimized using cyclodextrin chiral selectors. The influences of types of cyclodextrins added to background electrolyte (native vs. functionalized neutral and charged cyclodextrins), concentration and pH, temperature, applied voltage and capillary dimensions on enantioresolution were studied. Benzothiazole derivatives of amino acids valine, leucine and phenylalanine were used for the analysis. The derivatives can be positively charged on the amino- group under acidic conditions. The addition of native -, - and -cyclodextrin in concentration of 10 mM to the phosphate background electrolyte with concentration of 20-100 mM and pH in the range of 2.0-5.0 was tested. The enantiomeric purity of benzothiazole derivatives of L,D-phenylalanine, L,D-valine and L,D-leucine was verified in the concentration ratio from 1:4 to 1:100 (m/m).

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