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Determination of Ascorbic Acid in Pharmaceutical Preparation and Fruit Juice Using Modified Carbon Paste Electrode
Authors: Žabčíková Simona | Vu Dai Long | Červenka Libor | Tambor Vojtěch | Vašatová Martina
Year: 2016
Type of publication: článek v odborném periodiku
Name of source: Potravinarstvo
Page from-to: 255-259
Titles:
Language Name Abstract Keywords
cze Stanovení kyseliny askorbové ve farmaceutickém preparátu a ovocné šťávě s využitím modifikované uhlíkové pastové elektrody V této praci byla pro stanovení kyseliny askorbové použita uhlíková pastová elektroda modifikovaná barvivem (briliantová krezyl modř) a uhlíkovými nanotrubičkami. Barvivo působí jako mediátor zlepšující přenos elektrnů, zatímco uhlíkové nanotrubičky zvětšují aktivní plochu elektrody. Modifikovaná elektroda vykazovala dobrou elektrokatalytickou aktivitu pro oxidaci kyseliny askorbové při potenciálu 100 mV. Amperometricky byla zjištěna lineární odezva oxidace kyseliny askorbové v rozsahu koncentrací 0,1 – 350 µmol.L-1 s limitem detekce 0,05 µmol.L-1 a limitem kvantifikace 0,15 µmol.L-1. Tato metoda byla aplikována na analýzu obsahu kyseliny askorbové v famraceutickém preparátu a ovocném džusu. Výsledky získané s využitím modifikované uhlíkové elektrody byly v souladu s výsledky HPLC analýzy. kyselina askrobová; uhlíkové nanotrubice; amperometrie; brilintová modř
eng Determination of Ascorbic Acid in Pharmaceutical Preparation and Fruit Juice Using Modified Carbon Paste Electrode Ascrobic acid is key substance in the human metabolism and the rapid and accurate determination in food is of a great interest. Ascorbic acid is an electroactive compound, however poorly responded on the bare carbon paste electrodes. In this paper, brilliant cresyl blue and multi-walled carbon nanotubes were used for the modification of carbon paste electrode. Brilliant cresyl blue acts as a mediator improving the transition of electrons, whereas multiwalled carbon nanotubes increased the surface of the electrode. Both brilliant cresyl blue and multiwalled carbon nanotubes were added directly to the composite material. The electrochemical behavior of modified electode was determined in electrolyte at various pH, and the effect of the scan rate was also performed. It was shown that the electrochemical process on the surface of the modified carbon paste electrode was diffusion-controlled. The resulted modified carbon paste electrode showed a good electrocatalytic activity towards the oxidation of ascorbic acid at a reduced overpotential of +100 mV decreasing the risk of interferences. A linear response of the ascorbic acid oxidation current measured by the amperometry in the range of 0.1 – 350 µmol.L-1 was obtained applying the sensor for the standard solution. The limit of detection and limit of quantification was found to be 0.05 and 0.15 µmol.L-1, respectively. The novel method was applied for the determination of ascorbic acid in pharmaceutical vitamin preparation and fruit juice, and the results were in good agreement with the standard HPLC method. The presented modification of carbon paste electrode is suitable for the fast, sensitive and very accurate determination of ascorbic acid in fruit juices and pharmaceutical preparation. ascorbic acid; carbon nanotubes; amperometry; brilliant cresyl blue

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