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Easy to use two-dimensional liquid chromatography on Agilent 1290 Infinity 2D-LC Solution

Authors: Hájek Tomáš

Year: 2016

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Easy to use two-dimensional liquid chromatography on Agilent 1290 Infinity 2D-LC Solution	Two dimensional liquid chromatography (2D-LC) is a powerful technique for the separation of samples containing up to a few tens of compounds. 2D-LC allows increase in peak capacity, selectivity and resolution in comparison to a single dimension HPLC, especially in comprehensive LC×LC mode. In comprehensive LC×LC, the columns in the first and in the second dimensions are coupled via an interface with two equivalent sampling loops or trapping columns, operating in alternating cycles in which subsequent aliquot fractions of the first-dimension effluent are sampled and subsequently released in into the second-dimension system for secondary separation [1]. The basic premise for the use of 2D-LC is combine different separation mechanisms, such as reversed-phase (RP), ionexchange (IEX), and normal-phase (NP) or hydrophilic interaction (HILIC) chromatography, to accomplish an orthogonal retention with the maximum peak capacity in the time available for the analysis in two-dimensional LC×LC separation systems. Furthermore, the overall 2D peak capacity, the orthogonality of 2D system, and the coverage of separation space can be influenced by type of gradient runs in the both dimensions of a LC×LC system [2]. Four types of second dimension gradients may be used for achieve the best coverage of the separation space: full gradient, segment gradient, shift gradient and parallel gradient. A combination of HILIC in the first dimension and RP-LC in the second dimension provides good orthogonality for compounds such as phenolic acids and flavonoids in LC×LC setup. Phenolic acid and flavonoid compounds are naturally occurring aromatic secondary plant metabolites. More than 4000 flavones have been identified and divided into several subclasses with various structures. LC×LC is a powerful technique for the separation of these compounds	Two-dimensional liquid chromatography; LCxLC; second dimensional gradient; Agilent 2D-LC; HILIC; RP