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Determination of distribution constants of antioxidants between liposomes, alkylsulfate micelles, octanol and water using electrokinetic chromatography
Authors: Váňová Jana | Liimatta Laura J | Česla Petr | Wiedmer Susanne
Year: 2016
Type of publication: článek ve sborníku
Name of source: CECE 2016 : 13th International Interdisciplinary Meeting on Bioanalysis
Publisher name: Ústav analytické chemie AV ČR, v. v. i.
Place: Brno
Page from-to: 62-65
Titles:
Language Name Abstract Keywords
cze Stanovení distribučních konstant antioxidantů mezi liposomy, micelami alkylsulfátů sodných, oktanolem a vodou s využitím elektrokinetické chromatografie In vivo efekty antioxidantů jsou závislé na jejich lipofilicitě, která určuje interakce s proteiny a membránami. Lipofilicita antioxidantů byla studována s využitím dvou elektrokinetických systémů micelární elektrokinetické chromatografie (MEKC) a liposomální elektrokinetické chromatografie (LEKC). Pseudostacionární fáze sestávaly z micel decylsíranu sodného (SDeS) a dodecylsíranu sodného (SDS) a v LEKC ze směsí 1-palmitoyl-2-oleyl-sn-glycerofosfatidylcholinu (POPC) a 1-palmitoyl-2-oleoyl-sn-fosfatidylserinu (POPS) nebo 1-palmitoyl-2-oleoyl-sn-glycero-3-fosfatidyl-DL-glycerolu (POPG). Retenční faktory studovaných látek byly stanoveny při pH 7,4. Distribuční konstanty studovaných antioxidantů mezi micelami, liposomy a vodnou fází byly experimentálně určeny a porovnány s distribučními konstantami mezi oktanolem a vodou.
eng Determination of distribution constants of antioxidants between liposomes, alkylsulfate micelles, octanol and water using electrokinetic chromatography The in vivo effects of the antioxidants are dependent on their lipophilicity, which govern membrane and protein interactions. Two electrokinetic systems, i.e. micellar electrokinetic chromatography (MEKC) and liposome electrokinetic chromatography (LEKC), were used for studying of the lipophilicity of antioxidants. Micelles of sodium decyl sulfate (SDeS) and sodium dodecyl sulfate (SDS) were used as the pseudostationary phase in MEKC and the liposomes were composed of mixtures of 1-palmitoyl-2-oleyl-sn-glycerophosphatidylcholine (POPC) and 1-palmitoyl-2-oleoylsn-phosphatidylserine (POPS) or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyl-DLglycerol (POPG) in LEKC. The retention factors of the studied analytes were determined at pH 7.4. The micelle and liposome/aqueous distribution constants for studied antioxidants were experimentally determined and compared with values of octanol/aqueous distribution constants. distribution constants; antioxidants; liposomes; alkylsulfate micelles; electrokinetic chromatography

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