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Retention behavior of lipids in reversed-phase ultrahigh-performanceliquid chromatography-electrospray ionization mass spectrometry
Authors: Ovčačíková Magdaléna | Lísa Miroslav | Cífková Eva | Holčapek Michal
Year: 2016
Type of publication: článek v odborném periodiku
Name of source: Journal of Chromatography A
Page from-to: 76–85
Titles:
Language Name Abstract Keywords
cze Retenční chování lipidů s použitím kapalinové chromatografie s reverzními fázemi ve spojení s hmotnostní spekltrometrií s elektrosprejovou ionizací Tato práce se zabývá retenčním chování lipidů v biologických vzorcích jako je lidská plasma a moč a prasečí mozek. Pro práci byla použita kapalinová chromatografie s reverzními fázemi ve spojení s hmotnostní spektrometrií. Identifikace jednotlivých lipidů byla provedena v negativním a pozitivním módu s přesností lepší než 5 ppm. Bylo identifikováno 14 polárních a 5 nepolárních tříd a více než 400 jednotlivých lipidů. lipidy; kapalinová chromatografie; reverzní fáze; hmotnostní spekltrometrie; elektrosprejová ionizace
eng Retention behavior of lipids in reversed-phase ultrahigh-performanceliquid chromatography-electrospray ionization mass spectrometry Reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC) method using two 15 cmsub–2 m particles octadecylsilica gel columns is developed with the goal to separate and unambigu-ously identify a large number of lipid species in biological samples. The identification is performed by thecoupling with high-resolution tandem mass spectrometry (MS/MS) using quadrupole – time-of-flight(QTOF) instrument. Electrospray ionization (ESI) full scan and tandem mass spectra are measured inboth polarity modes with the mass accuracy better than 5 ppm, which provides a high confidence oflipid identification. Over 400 lipid species covering 14 polar and nonpolar lipid classes from 5 lipid cate-gories are identified in total lipid extracts of human plasma, human urine and porcine brain. The generaldependences of relative retention times on relative carbon number or relative double bond number areconstructed and fit with the second degree polynomial regression. The regular retention patterns inhomologous lipid series provide additional identification point for UHPLC/MS lipidomic analysis, whichincreases the confidence of lipid identification. The reprocessing of previously published data by our andother groups measured in the RP mode and ultrahigh-performance supercritical fluid chromatographyon the silica column shows more generic applicability of the polynomial regression for the description ofretention behavior and the prediction of retention times. The novelty of this work is the characterizationof general trends in the retention behavior of lipids within logical series with constant fatty acyl lengthor double bond number, which may be used as an additional criterion to increase the confidence of lipididentification. lipids; reversed-phase; ultrahigh-performanceliquid; chromatography–electrospray ionization; mass spectrometry

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