Login for students

Login for employees

Publication detail

Lipidomic analysis: comparison of supercritical fluid chromatography, liquid chromatography and direct-infusion mass spectrometry for analysis of lipids
Authors: Lísa Miroslav | Cífková Eva | Ovčačíková Magdaléna | Holčapek Michal
Year: 2016
Type of publication: ostatní - přednáška nebo poster
Page from-to: nestránkováno
Titles:
Language Name Abstract Keywords
eng Lipidomic analysis: comparison of supercritical fluid chromatography, liquid chromatography and direct-infusion mass spectrometry for analysis of lipids Lipidomics is a branch of metabolomics aimed at the full analysis of lipid species and their biological roles in the organism. Lipids play multiple roles in cellular functions. They form membrane bilayers, participate in the cell growth, multiplication, death and signaling processes. Due to their important functions in living organisms, they are considered as possible biomarkers of some serious human diseases, such as cancer, cardiovascular diseases, diabetes, etc. Lipidomic analysis is a challenging task for analytical chemists due to the extreme complexity of lipidomic samples given by numerous lipid species widely differing in their structures, polarities and concentrations. The aim of this work is a comparison of chromatography/mass spectrometry (MS) and direct-infusion MS based approaches for the lipidomic analysis, which are compared in one laboratory using identical analytical protocol and the same instruments. For this purpose, ultrahigh-performance supercritical fluid chromatography/electrospray ionization-MS (UHPSFC/ESI-MS) method based on 1.7 μm particle-bridged ethylene hybrid silica column and methanol/water/ammonium acetate modifier is used for the lipidomic analysis. Hydrophilic interaction liquid chromatography - ultrahigh-performance liquid chromatography (HILIC-UHPLC) is applied for the lipidomic analysis using the identical type of column and mass spectrometer enabling the direct comparison of results with UHPSFC method. Lipid class separation of lipids according to polarity is achieved by both methods with the identification of fatty acyl composition based on positive and negative-ion full-scan and tandem mass spectra measured with high mass accuracy and high resolving power. UHPSFC/ESI-MS method provides the separation of both nonpolar and polar lipids in one 6 minute analysis, while nonpolar lipids are not separated using HILIC-UHPLC/ESI-MS. lipidomic, LC, MS, SFC

Departments

Other Parts

Map

en.mapy.cz

Services

Popular Links

© 2023 University of Pardubice, Studentská 95, 532 10 Pardubice 2
Phone: +420 466 036 111, 466 036 112, 466 036 113
Webmaster, RSS
Data box ID: f5vj9hu
Accessibility Statement
CC BY-NC-ND 4.0Creative CommonsAttributionNonCommercialNoDerivatives