University of PardubiceFaculty of Chemical Technology

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Application of UHPSFC/ESI-MS method for the lipidomic analysis of kidney cancer tissue samples

Authors: Khalikova Maria | Lísa Miroslav | Cífková Eva | Vrána David | Melichar Bohuslav | Študent Vladimír | Holčapek Michal

Year: 2016

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Application of UHPSFC/ESI-MS method for the lipidomic analysis of kidney cancer tissue samples	idney cancer is the twelfth most common cancer in the world (joint position with pancreatic cancer), with 338 thousand new cases diagnosed in 2012 [1]. The determination of lipidomic differences is crucial for the improvement of cancer early diagnostic since the changes in lipids profiles are associated with cancer development and may be correlated to the disease stage. The ultrahigh-performance supercritical fluid chromatography (UHPSFC) technique brings benefits for the separation and analysis of polar and nonpolar lipid classes [2]. Optimized UHPSFC – electrospray ionization mass spectrometry (ESI-MS) method is applied for the characterization of changes in lipid profiles of kidney cancer tissue samples. 422 lipid species are identified and quantified in kidney tissues from 10 different lipid classes including cholesterylesters (CE), triacylglycerols (TG), diacylglycerols (DG), monoacylglycerols (MG), phosphatidylethanolamines (PE), lysophosphatidylethanolamines (LPE), ceramides (Cer), phosphatidylcholines (PC), lysophosphatidylcholines (LPC) and sphingomyelins (SM). The quantitation of lipids using UHPSFC/ESI-MS is based on peak abundances of corresponding ions in lipid class mass spectra after the isotopic correction related to the peak abundance of one internal standard per each lipid class.