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Lipidomic analysis of kidney cancer samples using UHPSFC/ESI-MS method

Authors: Khalikova Maria | Lísa Miroslav | Cífková Eva | Vrána David | Melichar Bohuslav | Študent Vladimír | Holčapek Michal

Year: 2016

Type of publication: ostatní - přednáška nebo poster

Page from-to: nestránkováno

Titles:

Language	Name	Abstract	Keywords
eng	Lipidomic analysis of kidney cancer samples using UHPSFC/ESI-MS method	Kidney cancer is of particular relevance in Europe caused by high incidence rates and increasing cases in most European countries in recent decades, especially in the Czech Republic having the highest incidence rates [1]. The changes in lipids profiles are associated with cancer development in the human organism and may be correlated to the stage of disease [2]. In this context, the determination of lipidomic differences is crucial for the improvement of cancer early diagnostic. The use of ultrahigh-performance supercritical fluid chromatography (UHPSFC) brings the unique benefits for the separation and analysis of polar and nonpolar lipid classes [3] and is applied in this work for the characterization of statistically significant changes in lipid profiles of kidney cancer samples. Optimized UHPSFC – electrospray ionization mass spectrometry (ESI-MS) method allows the separation and analysis of polar and nonpolar lipid classes in normal and cancer kidney tissues [3]. More than 400 lipid species are identified and quantified in kidney tissues from 10 different lipid classes including cholesterylesters (CE), triacylglycerols (TG), diacylglycerols (DG), monoacylglycerols (MG), phosphatidylethanolamines (PE), lysophosphatidylethanolamines (LPE), ceramides (Cer), phosphatidylcholines (PC), lysophosphatidylcholines (LPC) and sphingomyelins (SM). The quantitation of lipids using UHPSFC/ESI-MS is based on peak abundances of corresponding ions in lipid class mass spectra after the isotopic correction related to the peak abundance of one internal standard (IS) per each lipid class.